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資料種別 記事・論文

大腸菌WP2uvrA株を用いた突然変異スペクトル解析系の開発

太田 敏博,赤沼 三恵,石原 啓美,北 陽子,島村 万紀,川口 泰史,時下 進一,志賀 靖弘,山形 秀夫

詳細情報

タイトル 大腸菌WP2uvrA株を用いた突然変異スペクトル解析系の開発
著者 太田 敏博
著者 赤沼 三恵
著者 石原 啓美
著者 北 陽子
著者 島村 万紀
著者 川口 泰史
著者 時下 進一
著者 志賀 靖弘
著者 山形 秀夫
別タイトル A set of tester strains for the determination of mutational specificity in E.coli WP2uvrA with and without pKM101
著者別名 Ohta Toshihiro
著者別名 Watanabe-Akanuma Mie
著者別名 Ishihara Hiromi
著者別名 Kita Yoko
著者別名 Shimamura Maki
著者別名 Kawaguchi Yasushi
著者別名 Tokishita Shin-ichi
著者別名 Shiga Yasuhiro
著者別名 Yamagata Hideo
出版年(W3CDTF) 1998-07-21
件名(キーワード) transition
件名(キーワード) transversion
件名(キーワード) mutational specificity
件名(キーワード) pKM101
件名(キーワード) E.coli WP2uvrA
件名(キーワード) transition
件名(キーワード) transversion
件名(キーワード) mutational specificity
件名(キーワード) pKM101
件名(キーワード) E.coli WP2uvrA
要約・抄録 Two sets of useful tester strains(WP3101-WP3106 and WP3101P-WP3106P)derived from E.coli WP2uvrA were constructed for the detection and classification of mutagens. Six kinds of F' plasmid(lacI^-, lacZ^-, proAB^+)in strains WP3101-WP3106 carried a different lacZ^- allele. Assays for transitions and transversions are based upon Lac^+ reversion of a specific mutation located within the lacZ^- gene on an F' plasmid in the strains. Plasmid pKM101 was further transferred to each strain to make a set of strains WP3101P-WP3106P. In any strain the trpE65(ochre)allele is available for the Trp^+ reversion assays which is commonly used for mutagenicity test with the WP2uvrA strain. Using the new tester strains, the mutational specificities of 1, 2-dibromo-3-chloropropane and cumene hydroperoxide were investigated. In the presence of S9mix, 1, 2-dibromo-3-chloropropane specifically induced G:C→A:T transitions. Cumene hydroperoxide induced two types of transversions, G:C→T:A detected in WP3104 and A:T→T:A detected in WP3105, with G:C→T:A predominating. In pKM101-carrying strains, all 6 types of base substitutions were induced by cumene hydroperoxide with different efficiencies. The frequency of predominant G:C→T:A transversions was not affected by the introduction of pKM101, while that of other types of transitions and transversions were markedly increased.
要約・抄録 Two sets of useful tester strains(WP3101-WP3106 and WP3101P-WP3106P)derived from E.coli WP2uvrA were constructed for the detection and classification of mutagens. Six kinds of F' plasmid(lacI^-, lacZ^-, proAB^+)in strains WP3101-WP3106 carried a different lacZ^- allele. Assays for transitions and transversions are based upon Lac^+ reversion of a specific mutation located within the lacZ^- gene on an F' plasmid in the strains. Plasmid pKM101 was further transferred to each strain to make a set of strains WP3101P-WP3106P. In any strain the trpE65(ochre)allele is available for the Trp^+ reversion assays which is commonly used for mutagenicity test with the WP2uvrA strain. Using the new tester strains, the mutational specificities of 1, 2-dibromo-3-chloropropane and cumene hydroperoxide were investigated. In the presence of S9mix, 1, 2-dibromo-3-chloropropane specifically induced G:C→A:T transitions. Cumene hydroperoxide induced two types of transversions, G:C→T:A detected in WP3104 and A:T→T:A detected in WP3105, with G:C→T:A predominating. In pKM101-carrying strains, all 6 types of base substitutions were induced by cumene hydroperoxide with different efficiencies. The frequency of predominant G:C→T:A transversions was not affected by the introduction of pKM101, while that of other types of transitions and transversions were markedly increased.
対象利用者 一般
資料の種別 記事・論文
掲載誌情報(ISSN形式) 09100865
掲載誌情報(URI形式) 環境変異原研究
掲載誌名 環境変異原研究
掲載巻 20
掲載号 2
掲載ページ 75-82

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