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- 要約等A protoplast transformation system for Bacillus subtilis AC819 with pUB110 plasmid DNA under high pressure of kanamycin (100......been developed by using extremely low concentration of lysozyme, 2.5 μg/ml, wi......ion efficiency of protoplasts. The optimum transformation of protoplasts was achieved under the conditions as follows : lysozyme concentration, 2.5 μg/ml; in......on period with lysozyme at 40.5 C, for...... C. A response of kanamycin-resi...... to the number of protoplasts reached 1.9 ×......0^4 per 370 ng of plasmid DNA, which corresp......gher than that of competent cell transformation.
- 件名Bacillus subtilis Protoplast Lysozyme
- 関連情報九州大学農学部紀要 || 36(1/2) || p23-28 Journal of the Faculty of Agriculture, K......shu University || 36(1/2) || p23-28 http://www.agr.kyushu-...
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