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デジタル記事
1991-03九州大学医療技術短期大学部紀要18p.1-9
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  • 要約等DNase I was purified from rat pancreatic juice by a procedure including DEAE-cellulose and phosphocellulose chromatographies, Sephadex G-75 gel filtration and concanavalin A-Sepharose affinity chromatography. The purified enzyme contained no detectable activities of acid DNase, acid or alkaline RNase, acid or alkaline phosphatase or nonspecif...... phosphodiesterase. The molecular weight of the enzyme was estimated to be 37,500 by Sephadex G-75 gel filtration. Its isoele......me required divalent cations and shows dual pH optima that are dependent on divalent cations : in the presence of Co2+, the opti...... 6.0 in 17 mM cacodylate-HCl buffer and in the...
  • 関連情報九州大学医療技術短期大学部紀要 || 18 || p1-9 Memoirs of Kyushu University Scool of Health Sciences || 18 || p1-9
  • 著者標目Anai Motoaki Eshima Natsuko Matsumoto Fujiko

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