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- 要約等...ate-of-the-art information on ge......ntiality screenings in a wide variety......ms, i.e. screening for protein-coding genes and oth...... collection begins with chapters employing CRISPR/Cas9-b......ers also delve into other techniques for exploring essential genes, such as bioinformatics methods. Written for t......hly successful Methods in Molecular Biol......ries, chapters include introductions to ......on troubleshooting and avoiding known pitfall......s and Genomes: Methods and Protocols ......archers attempting to strip genetics down to it...
- 内容細目...R Screens with Inducible Cas9 CR......SPR/Cas9 Screening to Identify C......ssential Genes in Human Cell Lines Target Ident......ll Molecules Using Large-Scale C......tagenesis Scanning of Essential ......on of a CRISPR Interference Syst...... Gene Analysis in the Fungal Pat......ential Genes Using Sequential CR......ethod that Combines CRISPR and A......ssential Genes in Various Vertebrate Cell Lines New Method f......Genomics Study in Bacteria with ......rmance: CRISPR Interference Scre......d for Constructing Conditional M......ssential Genes in Cyanobacteria ...... of Transposon Insertion Sequencing in Comprehensively Identifyi...
- 件名Genes Genomics Gene editing Gènes Génomique Édition génique Gene editing. Genes. Genomics.
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- 要約等"This Handbook of Research Methods in Careers serves......esearchers use in career scholarship. Presenting detailed over......ce of research in the discipline. Chapters sho......se diverse and interdisciplinary approaches to studying careers acros......ive, and mixed methods. Providing an in-depth illustra......of established methods and current trends in careers resear......his Handbook brings together top international aut...... choices. Offering cutting-edge methods from established and emerging experts, this......is crucial reading for scholars ......currently studying or wish to st...... be useful for institutions coordinating large ...
- 内容細目...er, John McMackin, Szu-Hsin Wu, Jose Aldo ......er decision making / Gregory Hen......y Yip 6. Designing and studying mentoring programs: Rev....... Quantitative methods 7. Text mining in career studies: Generating insights from uns......l tell: Conducting longitudinal research on ......ocial networks in contemporary c......ultilevel modeling for careers r......tive and mixed methods 11. Engaging grounded theo......careers: Attending to the relati......reenberg 12. Using in-depth interviews in careers resear......identities and institutions: The......antitative examination of metaph......l language use in career-life preparedness / Al...
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- 要約等...l research. Beginning with an assortment of methods for the isolat......the volume continues with method......oxicity, viral infection, and me......, as well as clinical applicatio......hly successful Methods in Molecular Biol......ries, chapters include introductions to ......on troubleshooting and avoiding known pitfall......er (NK) Cells: Methods and Protocols ......esearchers looking to contribute......of basic and clinical NK cell research
- 内容細目Isolation of Innate Lymphoid Cells from Murine Intestinal Lamina Propria Purif......ional Analysis In Vitro Developm......ogenitor Cells Induction of Huma......Cells under Defined Conditions b......nsition from Maintenance Culture......odels for Studying Human NK Cells in Health and Dis...... Conjugation Using a SPAAC Coppe......10x Genomics' Single-Cell RNA-Seq Methods to Analyze the......ary NK Cells Using Monocle and SCENIC Analyses Methods for Isolating and Defining Single-Cell Transc......ic Color-Barcoding for Fate Mapping of Single Innate Lymphocytes Quantifying Human Natural......gration by Imaging and Image Analysis Tracking ...
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2463 Springer protocols (Series)
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- 要約等...d carrier protein dependent synthases, including polyketide sy......mistry, and bioinformatics. Written in the format of ......hly successful Methods in Molecular Biol......, each chapter includes an introduction to t...... materials and methods, includes tips on troubleshooting and known pit......tative and cutting-edge, Non-Rib......nthesis and Engineering: Methods and Protocols ......ims to feature methods that will be b...... and those wanting to adopt new methodologies into their research
- 内容細目The Assembly-line Enzymology of......sis Structural Studies of Modular Non...... Synthetases Using NMR Titration to Study Protein-Protein Interactions in AcpP Dependent......roteomic Profiling of Adenylation Domain Functions in Gramicidin S-producing Nonribosomal ......de Synthetases In Vitro Biochemi......sed Macrocyclizing Thioesterase Domains from Non-Ribo......s by a Penicillin-binding Protein-type Thioester......A Carrier Protein Domain for Recognitio......Phosphopantetheinyl Transferases to Enable Inhibitor Screening Unraveling Structural Information of Multi-Domain Nonribosomal P......nthetases by Using Photo-Crosslinking Analysis ...
- 一般注記Includes index
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- 要約等.... The chapters in this book cove......ted DNA sequencing; target enrichment sequencing via probe cap......transformation methods; prediction of long non-coding RNAs and seco......; genome-wide mining of disease re......gs; genome editing, and bioactiv......lysis. Written in the highly successful Methods in Molecular Biol......rmat, chapters include introductions to ......on troubleshooting and avoiding known pitfalls. Cutting-edge and informative, Legume Genomics: Methods and Protocols ......ert researcher involved in various molecu...... general plant studies. --
- 内容細目... Genome Sequencing Updates on Ge......omic Resources in Chickpea for C......Construction using an Iterative Mapping and Assembly Approach Methods and Tools for ...... Genome Sequencing, Assembly and......tream Analysis Methods of Gene Expression Profiling to Understand......n and Response in Legume Crops M......ulphite Sequencing Data Analysis......tion Detection in Legumes Method......Example Genotyping for Species I......y Assessment using Double-Digest......ted DNA Sequencing (ddRAD-seq) F......omic Selection in Crop Breeding Methods for Target Enrichment Sequencing via Probe Capture in Legumes Developing Transposable Element Marker ...
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2107 Springer protocols (Series)
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- 要約等...ers on longitudinal studies, randomized tr......d decisions making, and a new section on changing health-relate....... The chapters in this book are organized into six parts: P......focuses on framing clinical research q......tions and choosing a suitable de......that may occur in clinical research; ......ociated with doing conducting research on h......ysis that pertain to evaluation of risk in longitudinal studies; assessment of therapy in controlled tri...... Five presents methods used in various compon......ed decision-making; and Part Six highlights interventions focused on changing health-relate......viors. Written in the highly successful Methods...
- 内容細目...rials 6: Determining the Sample Si......nd Power for Clinical Trials and Cohort Studies Randomized Con......ls 7: On Contamination and Estimating the Actual Tr......ative Research in Clinical Epidemiolo......ed Decision Making 1: Critical A......ed Decision Making 2: Systematic......ed Decision Making 3: Health Tec......ed Decision Making 4: Clinical Practice Guidelines Evidence-Based Decision Making 5: Knowledge ......ed Decision Making 6: Administrative Datab......rvice Research Methods in Evidence-Based Decision Making 7: Health Economics in Clinical Research Methods in Evidence-Based Decision Making 8: A Primer for Researchers ...
- 件名... Epidemiologic Methods Research Design Evidence-Base...
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- 要約等...ppression, editing, and reprogramming; cardiac gene...... gene delivery methods; pulmonary hyp...... patient screening and measuring the efficacy ......erapy. Written in the highly successful Methods in Molecular Biol......rmat, chapters include introductions to ......on troubleshooting and avoiding known pitfalls. Cutting-edge and comp...... Gene Therapy: Methods and Protocols,......or researchers in cardiology who are conducting gene therapy ......d successful clinical translations in the field
- 内容細目...y Research and Methods: Overview of C......rect Reprogramming of Adult Huma......ac Fibroblasts into Induced Cardiomyocytes using miRcombo CRIS....../Cas9 Gene Editing of RYR2 in Human iPSC Der......obe Ca2+ Signaling Aberrancies o......ogenesis Enhancing Cardiomyocyte......ranscription using In Vivo CRISPR/Ca......matic Gene Editing for Cardiac a......keletal Muscle in a Large Animal......ic Construct using Sarcolipin Promoter Assoc......sociated Virus Injection in Rats Cardiac Gene Delivery in Large Animal M...... Gene Delivery in Large Animal M......rograde Venous Injection Endocar......ene Delivery using NOGA Catheter System Surgica...
- 件名...netic Therapy--methods Heart Diseases--therapy Gene ...
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- 要約等...mentation pertaining to chemistry,......gy of CCN proteins. The chapters in this book cove......roarray; utilizing public molecu......says for measuring bone remodeling activities of CCN proteins; and the function of CCN2 in tubular epithe......nesis. Written in the highly successful Methods in Molecular Biol......rmat, chapters include introductions to ......on troubleshooting and avoiding known pitfalls. Cutting-edge and comp......sive, CCN Proteins: Methods and Protocols,......esearchers looking for new appro......hers just starting out in the field of CCN research
- 内容細目CCN Proteins - Cellular Co......Factors: Expanding their Reperto......uorescence Imaging for the Evalu......CN Family Proteins- Imaging of Molecular Interaction between CCN Protein and Its Binding Partners: An In Situ Proximity......ation Assay of Interaction Betwe......CCN2 and Rab14 in Chondrocytes W......is of CCN Proteins and Fragments in Exosomes, Vesi......of CCN Factors in/on Exosomes Ev...... the Molecular Interaction between CCN Protein and Its Binding Partners: A Solid-Phase Binding Assay and Sur......cols for Screening Peptides Binding to CCN Family Proteins and their Ext......ransfection, Spinfection, Exofection, and Lucif...
- 件名Cysteine--Laboratory manuals Protein-protein interactions--Lab......y manuals Cysteine. Protein-protein interactions.
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- 要約等...e provides the increasing number of SAR...... handbook covering multidisciplinary approaches ......ogether by leading laboratories ......Topics covered include techniques in clinical and diagno......asic protocols in cell and virus...... as well as bioinformatics and p......ics approaches in cellular response studies. This comprehe......on also covers methods in immunology, an......virals and vaccine development s......hly successful Methods in Molecular Biol......ries, chapters include the kind of detailed i......al for success in the lab. Pract......y, SARS-CoV-2: Methods and Protocols ......for scientists investigating this prevalent and...
- 内容細目...udy Virus-Host Interactions During SARS-CoV-2 Infection Human N...... by Air-Liquid Interface (ALI) C......ystem for Studying the Pathogene...... Antigens, by Single and Multipl......hemistry Measuring Neutralizing Antibodies to SARS-CoV-2 Using Lentiviral Sp...... Strategies Against SARS-CoV-2: ......for SARS-CoV-2 Infection: Plaque......sed Drug Screening Assay for SAR......oV-2 Strengthening Biorisk Management in Research Labor......CoV-2 Research in a Biosafety Le...... Core Facility Methods of SARS-CoV-2 Inactivation
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2452 Springer protocols (Series)
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- 要約等... of up-to-date methods in the fields of ......ics. Organized into four major s......the volume examines methods utilizing model animal ......nd amphibians, methods using plants to stu......toxicity, applying the Ames assa......dy, as well as methods for environmen......ook is divided into these parts, the methods can be used ac......hly successful Methods in Molecular Biol......ries, chapters include introductions to ......on troubleshooting and avoiding known pitfall......icogenomics: Principles, Methods, and Applicati......uate students, inspiring more research in the vitally important field o...
- 内容細目...poptosis Following Exposure to E......onmental Contaminant Mixtures: A......Metal Mixtures in Caenorhabditis......tal Pollutants-Induced Mitochond......ial Toxicity Using Caenorhabditi......a Model System Methods to Assay the B......Toxicity Study Investigating the Joint Effects of Pe......et-B Radiation in Xenopus laevis......llutants Employing Multiple New ......eurobiological Methods to Investigate Envir......eurotoxicology in Mice Analyses ......c Modification in Environmental Pollutants-Induced Neurotoxi......s Technologies in the Research o......eath Practical Methods and Technologies in Environmental Epidemiology In...
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2326 Springer protocols (Series)
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- 要約等...chapters detailing a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins with their na......rugs or other binding partners. Cha......tive understanding of the mechanism of interaction, and ......iques. Written in the highly successful Methods in Molecular Biol......rmat, chapters include introductions to ......on troubleshooting and avoiding known pitfall......cessible, Protein Ligand Interactions: Methods and Applicatio......zation of protein interactions
- 内容細目Assessing and Improving Protein Sample Quality...... Familiar Protein-Ligand Interaction Revis...... with Multiple Methods Interactions of a......nsduction Protein Investigated by F...... Stopped-Flow Kinetics Kinetic Methods of Deducing Binding Mechanisms Involving Intrinsically Disordered Proteins Isothermal Ti......orimetry Measuring the Kd of Protein-Ligand Interactions Using Microscale Th......ipation Monitoring (QCM-D); Preparing Functionalize......f Complex Protein-Ligand Interactions Indirect Detection of Ligand Binding by Thermal Me......roughput Screening Ligand Discov......h Through-put Binding: Fluorescence Polarizatio...
- 件名Proteins--Laboratory m......y manuals Protein binding--Laboratory manuals Ligand binding (Biochemistry......anuals Ligand binding (Biochemistry...... Ligands. Protein binding. Proteins.
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- 要約等"Studying the role of music within religious cong...... has become an increasingly complex exe......ant variations in musical style ......ons require an interdisciplinary methodology......hile also allowing for nuance in interpretation. T......elp scholars think through the complexities of interdisciplinary research on......ional music-making by critically examining the theories and methods used by leading scholars in the field. An international and interdisciplinary panel of contributors introduce readers......hodologies within the emerging field of Cong......gational Music Studies. Utilising insights from fields such as com...
- 内容細目...ve it": addressing whiteness in congregational voicing / Marissa Gly...... Moore Researching Black congreg...... a migratory point of view: methods, challenges, a......rategies / Melvin L. Butler Studying Byzantine Ukrainian congregational music in Canada: considering community and......ongregational singing and practices of gender in Christian worship: exploring intersections / T......a Berger Searching for a metapho......cclesioscapes: interpreting gatherings around Christian music in and outside th...... case of the "Sing along Matthäuspassion" / Mi...
- 関連情報Congregational music studies series
紙図書児童書
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- 内容細目...能統合型指導 A Utilizing 幕末～明治期のドイツ語教育......ls common teaching method スティーブン......開拓 辰己明子 A teaching method for al......リシャン・クマー Utilizing social media ......ocratic method in the English language classroo...
- 典拠情報（件名/「を見よ」参照）Language and languages--Study and teaching
- 並列タイトル等（連結）Frontier of foreign language teaching methods
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- 要約等...chnologies and methods--combined with new gen......ools available in animal models--used in this constantly evolving field. The chapters in this book are organized into three sectio......ee explores bioinformatics and imaging analysis for ......cells. Written in the highly successful Methods in Molecular Biol......rmat, chapters include introductions to ......on troubleshooting and avoiding known pitfalls. Cutting-edge and comp...... Regeneration: Methods and Protocols ......esearchers looking to expand the......egeneration, aging, and regenerative medicine
- 内容細目...lation, and Staining of Murine Muscle Satell......ell Colony Forming Cell Assay as...... from Regenerating Skeletal Muscle Measuring Oxygen Consum......on Rate (ECAR) in Muscle Stem Cells Using a Seahorse An......cability for Aging Studies High Throughput Screening of Mitochondr...... Bioenergetics in Myoblasts and ......al Progenitors in Skeletal Muscl......vated Cell Sorting and Tissue Clearing In Vitro Maturati......tem Cells from Induced Pluripote......em Cells Producing Engraftable S......Techniques for Injury, Cell Tran......gical Analysis in Skeletal Muscle Murine Models of Tenotomy-Induced Mechanical Overloading a...
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2640 Springer protocols (Series)
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- 内容細目Front Cover Series Page Methods in ENZYMOLOGY Cop...... of dihydrouridine modifications in RNA 1 Introduction 1.1 ......A function 1.2 Methods to detect dihydrouridine 2 Step-by-ste......A) selection using oligo (dT) ce......A) selection using oligo (dT) ma......ze selection using silica column......3.3 3′ End healing 3.4 RNA size ......lysis 4.1 Trimming of adapter se......plicate collapsing 4.3 UMI remov......l 4.4 Read mapping 4.5 Read end position gathering 4.6 D-site id......ion 5.2 Sequencing depth and bio......olution sequencing technology for mapping pseudouridines in mammalian mRNA......olution sequencing technology 2 Materials 2.1 P...
- 関連情報Methods in enzymology
- 掲載誌Methods in enzymology
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- 内容細目Front Cover Series Page Methods in Enzymology Cop......ructured RNA 1 Introduction 2 General methods of RT-PCR using MarathonRT 2....... Before you begin 2.2 Key resour......ls 2.4.1 Annealing RT primers to......es 2.4.2 Preparing the reverse t......ure 2.4.3 Carrying out the rever...... 2.4.4 Programming a thermal cyc......er 2.4.5 Preparing the PCR mixture 2.4.6 Carrying out the PCR a......tion 2.4.7 Examining the PCR produ......nd troubleshooting 2.8.1 Potenti......ons for optimizing the procedure...... Sanger sequencing of RNAs containing complex RNA r......ents 2 General methods of RNA Sanger sequencing using MarathonRT 2.2 Key res...
- 関連情報Methods in enzymology
- 掲載誌Methods in enzymology
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国立国会図書館全国の図書館
紙図書
国立国会図書館全国の図書館
- 要約等...gy, genetic engineering, and genome editing technologies. Dividing into three parts,...... as genetic engineering and tissue culture of rice, including efficient methods for rice trans......on, genome editing, targeted integration, and gene stacking in rice, including multiple methods utilizing CRISPR system......n via base editing, and diverse methods describing bioinformatic, molec......lular analyses in rice. Written ......hly successful Methods in Molecular Biol......ries, chapters include introductions to ......on troubleshooting and avoiding known pitfall......Rice Genome Engineering and Gene Editing: Methods and Protocols serve...
- 内容細目...genome editing in rice protoplasts using CRISPR/CAS9 construct Single transcript ......for genome editing in rice Improving a quantitative trait in rice by multigene editing with CRISPR-C......9 Rice haploid inducer developme...... by genome editing FLP-mediated ......-specific gene integration in rice Rice gene targeting by homologous recombination with posi...... start sites using TransPrise: A novel machine learning approach Single cell type s......ssion analysis in rice using laser capture......sis of C4 proteins in the leaf tissu......ene expression studies in rice Rice prot......nalyses of domains amplified in plant genomes: ...
- 件名Rice--Genetic engineering Rice--Molecul......ce--Genetic engineering.
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- 内容細目... and Siobhán McInerney-Lankford ......and science of interpretation in human rights law / Martin Scheinin Legal methodol......es / Siobhán McInerney-Lankford Core principles in argumentation and understanding : hermeneutic......e Bondevik and Inga Bostad Econo......ransnational : methods in the study of h......f ethnographic methods for human righ......ly Engle Merry Interdisciplinarity and multi...... A. Andreassen Inside the organization. Methods of researching human rights ...... Tomas Max Martin Quantitative methods in advocacy-orien...... and Daniel Kacinski Challenges ....../ Simon Walker Methods of monitoring the right to fo...
- 資料の内容に関する注記...been neglected in human rights r......legal scholars in particular tending to address research methods and methodolog......ook advances thinking on human righ......hodology, offering instruction and g...... research. Seeking to bridge the......pounded by the interdisciplinary nature of h......butions by leading scholars in a range of evolving fields, provi......f human rights methods. The various c......rs apply these methods to different s......stantive areas including discrimination, the righ......ment of disciplinary approaches,......ights research in the crossroads......cademic disciplines. Accessible and engaging, t...
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- 要約等...apeutics, covering therapeutics using antisense oli......nt, genome editing, small molecu......ten by leaders in the field, the...... are currently in use and are starting an exciting therapeutic revolution in muscular dystr......hly successful Methods in Molecular Biol......ries, chapters include introductions to ......on troubleshooting and avoiding known pitfall...... Therapeutics: Methods and Protocols ......al resource to inspire readers and provide tips...
- 内容細目...sen: From Preclinical Studies to FDA Approval Rapid Freezing of Skeletal a......diac Muscles Using Isopentane Co......etic, and Protein Expression Studies Cardiac and Sk......scle Pathology in the D2/mdx Mou......ation of Utrophin Physiological ...... Body Function in the Canine Model of Duch......ystrophy Restoring Dystrophin Expression by Skipping Exon 6 and 8 in Neonatal Dystr......hic Dogs Restoring Dystrophin Expression wit......44 and 53 Skipping in the DMD Gene in Immortalized Myotubes Restoring Dystrophin Expression wit......y Exon 45 Skipping in Induced-Pluripote......n of Exon Skipping in Urine-Derived Cells for D...
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2587 Springer protocols (Series)