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- 要約等...mentation pertaining to chemistry, biology, physiology, p......gy of CCN proteins. The chapters in this book cove......roarray; utilizing public molecular biological dat......says for measuring bone remodeling activities of CCN proteins; and the function of CCN2 in tubular epithe......nesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfalls. Cutting-edge and comp......sive, CCN Proteins: Methods and Protocols,......esearchers looking for new appro......hers just starting out in the field of CCN research
- 内容細目CCN Proteins - Cellular Co......Factors: Expanding their Reperto......uorescence Imaging for the Evalu......CN Family Proteins- Imaging of Molecular Interaction between CCN Protein and Its Binding Partners: An In Situ Proximity......ation Assay of Interaction Betwe......CCN2 and Rab14 in Chondrocytes W......is of CCN Proteins and Fragments in Exosomes, Vesi......of CCN Factors in/on Exosomes Ev......luation of the Molecular Interaction between CCN Protein and Its Binding Partners: A Solid-Phase Binding Assay and Sur......cols for Screening Peptides Binding to CCN Family Proteins and their Ext......ransfection, Spinfection, Exofection, and Lucif...
- 件名Cysteine--Laboratory manuals Protein-protein interactions--Lab......y manuals Cysteine. Protein-protein interactions.
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- 要約等...zed analytical methods on a wide rang......ipids. From sphingoglycolipids t......mulant present in lipopolysaccha......he book delves into how glycolip......een organisms. In addition, glyc......important role in infections in animals and ot......nisms by targeting host cell mem......nsidered as living organisms. Wr......hly successful Methods in Molecular Biology series, chapters include introduction to t......on troubleshooting and avoiding known pitfall......, Glycolipids: Methods and Protocols ......lipid research in an effort to help researchers...
- 内容細目... Synthesis and Molecular Interaction Analy......erial Lipid As Innovative Vaccine Strategy: Self-Adjuvanting Conjugate Vaccines Chemical Syn......truction of Sphingolipid Remodel...... by Genome Editing Mass Spectrom......eutral Glycosphingolipids Struct......he Glycolipids in Lipid Rafts Ex......Microbiota Strains NMR Analysis ......of Glycolipids in the Plasma Mem......pecific Probes in Combination with Sodi......e Replica Labeling (SDS-FRL) Bio......nalyses for Sphingolipids and It......ated Molecules in Phagosomes Single-Molecule Imaging of Ganglioside Probes in Living Cell Plasma M......ion of Enzymes Involved in Glycolipid Metabolis...
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2613 Springer protocols (Series)
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- 要約等This volume provides methods on different a...... multiplex cloning. Chapters cov......olic and signaling pathways, advanced engineering of metabolic ......organisms, and methods for the on com......bility of proteins. Written in the format of ......hly successful Methods in Molecular Biology series, each chapter includes an introduction to t...... and reagents, includes tips on troubleshooting and known pit......tative and cutting-edge, Plant Synthetic Biology: Methods and Protocols ......searchers starting to explore no......or experts willing to expand their portfolio of...
- 内容細目...plant terpenes in the photosynth......gn of multiplexing CRISPR/Cas9 c......lant genome engineering using the GoldenBra......for genetic engineering of cyanobacte......ed mutagenesis in plants Generat......robes Gene editing in green alga Chlamydomonas reinhardtii via CRI......ribonucleoproteins Coexpression ......matic cascades in bacteria using UbiGate Homo-FRET imaging to study protein-protein interaction and c......plex formation in plants Plant X......iptional units in plants AGROBES......ression system in Arabidopsis seedlings Genetically-......analysis of auxin dynamics in plant cells Exploiting the Gal4/UAS system as plant...
- 件名...tion Synthetic Biology Biologie synth......logy Synthetic biology
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- 要約等...te-of-the- art methods on computer-ai......eaders through information on an......ructures, modeling antibody stru......tibody-antigen interactions, and......ed antibody affinity maturation ......eyond. Written in the format of ......hly successful Methods in Molecular Biology series, each chapter includes an introduction to t...... and reagents, includes tips on troubleshooting and known pit......tative and cutting-edge, Compute......nd experts looking to expand their knowledge
- 内容細目...ure analyses using IMGTª: 30 years of immunoinformatics Struc......tion of CDR-H3 in Single-Domain VHH Antibodies......tational modelling of Antibody a......r (CDR3 loops) Molecular dynamics simulation for investigating antigen-antibody interaction Molecular Dynamics Methods for Antibody Design Probing antibody dyna......Estimation of Single Domain Antibodies Using Molecular Dynamics Simulations Assessing and engineering antibody stability using experimental ...... computational methods In Silico Predict......ethod for Protein Asparagine Deamidation S......ical Guide for Molecular Modelers B-cel...... predictions using computational methods Comput...
- 件名Immunoinformatics Immunoglobulins--Computer-aided design Immunoinformatics.
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- 要約等...e provides the increasing number of SAR...... handbook covering multidisciplinary approaches ......ogether by leading laboratories ......Topics covered include techniques in clinical and diagno......asic protocols in cell and virus...... as well as bioinformatics and p......ics approaches in cellular respo......on also covers methods in immunology, an......virals and vaccine development s......hly successful Methods in Molecular Biology series, chapters include the kind of detailed i......al for success in the lab. Pract......y, SARS-CoV-2: Methods and Protocols ......for scientists investigating this prevalent and perilous ...
- 内容細目...RS-CoV-2 Virus Molecular Epidemiology o......oV-2 by Sequencing Advanced Gene...... Methodologies in Tracking Evolution and......ntigen-Based Point of Care Testing (POCT) for Diagnosing SARS-CoV-2: Assessing Performance D......for COVID-19 Using Sugar Chain-Immobilized Na......ody Techniques in the Diagnosis of SARS-CoV-2 Infection in Humans Propaga......ntification of Infectious SARS-C......Tissue Culture Infectious Dose Endpoint Dilution Assa......fication Profiling SARS-CoV-2 Infection by High......pectrometry Bioinformatics-Based......udy Virus-Host Interactions During SARS-CoV-2 Infection Human N...... by Air-Liquid Interface (ALI) Culture as a Mod...
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2452 Springer protocols (Series)
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- 要約等... plant systems biology on three speci......ic scales: the molecular level, the tis......latory network inference and dynamic modeling using ordinary differentia......ls for at-will induction of plan......hematical modeling of plant tissue using SimuPlant or ......af; and simulating crop root systems using OpenSimRoot o......-SWMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfall......ensive and cutting-edge, Plants Systems Biology: Methods and Protocols,......biologists looking for different......approaches to finding solutions to their questi...
- 内容細目...rench Profiles Investigating Soil-Root Interactions with......SWMS How to Define the Appropria......ments When Solving Water Flow in Root System Hy......ctures? Simulating Crop Root Systems using OpenSimRoot F......ure Challenges in Plant Systems Biology Plant Systems Biology: Further Reading and Resources
- 件名Plant molecular biology Systems biology Systems Biology Biologie moléculaire végét...
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- 要約等... of up-to-date methods in the fields of ......al toxicology, molecular toxicology, an......ics. Organized into four major s......the volume examines methods utilizing model animal ......nd amphibians, methods using plants to stu......toxicity, applying the Ames assa......dy, as well as methods for environmen......ook is divided into these parts, the methods can be used ac......hly successful Methods in Molecular Biology series, chapters include introductions to ......on troubleshooting and avoiding known pitfall......icogenomics: Principles, Methods, and Applicati......uate students, inspiring more research in the vitally...
- 内容細目...eath Practical Methods and Technologies in Environmental Epidemiology In Ovo Early-in-Life Inhalation Exposu......del DNA Damage in Liver Cells of......sambicus Larva Induced by the Insecticide Cyant......ethal Doses During Chronic Expos...... Nanoparticles in Plant Determination of Oxidat......ogical Phenotyping During Heavy Metal E......rotocol for Engineered Nanomater......dmium Toxicity in Plant Mutageni......due Pesticides in Food and Water......e Pesticides Using Gas Chromatog......trometry Determination of the N-......roso Compounds in Mouse Following RDX Exposure Determination of Metal Content in Drosophila melanogaster Durin...
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2326 Springer protocols (Series)
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- 要約等... comprehensive methods and protocols ......e grouped by main topic, starting with methodologies describing library prepa......tion and screening, state of the......art techniques in directed evolu......nzymes on sustainable polymers, ......hly successful Methods in Molecular Biology series, chapters include introductions to ......on troubleshooting and avoiding known pitfall......ely, Enzyme Engineering: Methods and Protocols ......ran researcher interested in biocatalysis. Chapter 13 is a...
- 内容細目...ries and Screening for Gene Spec......roughput Screening of Metagenomic Libraries Using Droplet Micro......aries for Protein Engineering toward Process Improvement in Drug Synthesis In Silico Prediction Methods for Site-Satur......agenesis Recombination of Compat......by 2GenReP and InSiReP Using the Evolution......istory of Proteins to Engineer Insertion-Deletio......al Templates Using Graphical Rep......RASP) Resurrecting Enzymes by An......Expression and In Vivo Loading of De Novo Proteins with Tetrapyr......onal-Design Engineering to Improve En......rmostability Using Molecular Simulation to Guide Protein Engineering for Biocatalysis ...
- 件名...ology Enzymes--Industrial applications Protein engineering Enzymes--Biot......--Applications industrielles Tec......ogie des protéines Enzymes--Bio......logy. Enzymes--Industrial applications. Protein engineering.
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- 要約等...ttribution 4.0 International License via link.springer.com
- 内容細目...Probs-MPI Aligning Protein-Coding Nucleotide Se...... Computation using the T-Coffee ......ous Datasets using SATé, PASTA,......alysis of Protein Intermolecular Interaction with ......and Sister Web-Methods: A Practical Guide to Bioinformatic Analysis of Protein Superfamilies ......nce Alignment, Molecular Phylogenetic A......mission Revisiting Evaluation of......ence Alignment Methods
- 件名Sequence alignment (Bioinformatics) Bioinformatics Bioinformatics.
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- 要約等...ree genome editing technologies,......nimal. Written in the successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfall......tative and cutting-edge, Genome Editing in Animals: Methods and Protocols,......er their study in this field
- 内容細目...Evaluation of Zinc Finger Nucleases U...... SNPD-CRISPR: Single Nucleotide ......lymorphism-Distinguishable Repre......R System Utilizing large functio......by cytoplasmic injection of CRIS......RNA Gene targeting in mouse embryoni......ribonucleoprotein (RNP) mediated genome editing Generation of Knock-in Mouse by Genome Editing Introduction of g......etic mutations into mice by Base......AID Genome editing in mouse and rat ......of flox mice using in vitro Cre recombination VCre/Vlox......specific recombination systems for genome engineering Efficient CRI......-Assisted Knockin of Large DNA D......ronuclear Microinjection During S-phase in Mous...
- 件名Animal genetic engineering Transgenic animals Gene editing Genetics--Tec......mal genetic engineering. Gene editing. Genetics--Technique. Transg...
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- 要約等...d of tissue engineered vascular ....... The chapters in this book cove......s such as deriving vascular cell...... monocytes and induced pluripote......stem cells; engineering vascular grafts using various bioma......vascular bioengineering technologies ......uch as 3D bioprinting; and fabricat......ocols for grafting and evaluatio......ascular grafts in animal models, vascular imaging in animals, and t......ility. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfalls. Cutting-edge and prac......ular Tissue Engineering: Methods and Protocols ...
- 内容細目...erry Silk Proteins Fabrication a......n of Tissue-Engineered Vascular ......ucture Controlling Pore Size of ......by Electrospraying of Poly(Ethyl......Microparticles Injectable Hydrog......ular Tissue Engineering Rabbit Surger......s Vascular Imaging in Small Animals Using Clinical Ultrasound......Implantation using a Bilateral E......ortoiliac Preclinical Model Quantification of In Vitro Blood-Brain Barrier Permea......helial Cells using Silicone Pill......s Vortices within the Cerebral Vasculature using Information Entropy
- 件名Vascular grafts Tissue engineering Blood vessel ......logy Tissue Engineering Blood Vessel ......Vaisseaux sanguins--Croissance Vaisseaux sanguins--Histologie B......wth. Tissue engineering. Vascular grafts.
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- 要約等...s applications in molecular biological tec......tions to determine the involvement of glycans in virus interactions. Cha......glycan and lectin microarray, pr......ation of recombinant viral domain protein, reverse genet...... and receptor binding, virus-host interactions and receptor binding, and sialidase: assays and inhibitors. Written in the successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfall......tative and cutting-edge, Glycovirology: Methods and Protocols aims to be an e...
- 内容細目...r glycan profiling of glycoproteins and glycosphingolipids by glycoblotting and BEP methods High-performan......matography mapping method for th......glycans at the molecular, cellular, and......cell surface using flow cytometry Lectin Staining to Detect Human and Avian influenza Virus Receptors in the Airway of ......lycan Arrays Using Glycopeptides......s Glycan profiling of viral glycoproteins with the lectin microarray Pre......ation of recombinant PA endonuclease domain protein of influenza A virus......ation for glycobiology research Receptor-binding specificity of influenza viruses...... of sulphatide in influenza A virus infection a...
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2556 Springer protocols (Series)
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- 要約等...sections detailing modification ......f microtubules in vitro, develop......f microtubule-binding molecules, co......f microtubules in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfall......tative and cutting-edge, Microtubules: Methods and Protocols ......provide useful information for construction of ...
- 内容細目Purification of Tubulin from Porcine Brain and its Fluore......Method of Tubulin Purification from Goat Brain for Reconstitu......ule Associated Intracellular Fun......zation of Tubulin: Approaches to Modify Tubulin with Biotin and DNA Electr......lation of Tubulin Properties and Function In Vitro Reconsti......amics and Severing Imaged by Label-Free Interference Refl......opy Characterizing the Number of Kinesin Motors Bound to Microtubules in the Gliding Motility Assay using FLIC Microsco...... Microtubules Linear-Zero Mode Waveguides for Single-Molecule Fl......of Nucleotides in Kinesin-Microtubule Mo......d Quantum Dots Integration Leads to Conjugates ...
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2430 Springer protocols (Series)
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- 要約等...apeutics, covering therapeutics using antisense oli......nt, genome editing, small molecu......ten by leaders in the field, the...... are currently in use and are starting an exciting therapeutic revolution in muscular dystr......hly successful Methods in Molecular Biology series, chapters include introductions to ......on troubleshooting and avoiding known pitfall...... Therapeutics: Methods and Protocols ......al resource to inspire readers and provide tips...
- 内容細目...sen: From Preclinical Studies to......val Rapid Freezing of Skeletal a......diac Muscles Using Isopentane Co......etic, and Protein Expression Stu......scle Pathology in the D2/mdx Mou......ation of Utrophin Physiological ...... Body Function in the Canine Model of Duch......ystrophy Restoring Dystrophin Expression by Skipping Exon 6 and 8 in Neonatal Dystr......hic Dogs Restoring Dystrophin Expression wit......44 and 53 Skipping in the DMD Gene in Immortalized Myotubes Restoring Dystrophin Expression wit......y Exon 45 Skipping in Induced-Pluripote......n of Exon Skipping in Urine-Derived Cells......phy Use of Glycine to Augment Exon Skipping and...
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2587 Springer protocols (Series)
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- 要約等...d carrier protein dependent synthases, including polyketide sy......gy, structural biology, proteopromics, chemical biology, natural produ......mistry, and bioinformatics. Written in the format of ......hly successful Methods in Molecular Biology series, each chapter includes an introduction to t...... materials and methods, includes tips on troubleshooting and known pit......tative and cutting-edge, Non-Rib......nthesis and Engineering: Methods and Protocols ......ims to feature methods that will be b...... and those wanting to adopt new methodologies into their research
- 内容細目The Assembly-line Enzymology of...... Synthetases Using NMR Titration to Study Protein-Protein Interactions in AcpP Dependent......roteomic Profiling of Adenylation Domain Functions in Gramicidin S-producing Nonribosomal ......de Synthetases In Vitro Biochemi......sed Macrocyclizing Thioesterase Domains from Non-Ribo......s by a Penicillin-binding Protein-type Thioester......A Carrier Protein Domain for Recognitio......Phosphopantetheinyl Transferases to Enable Inhibitor Screening Unraveling Structural Information of Multi-Domain Nonribosomal P......nthetases by Using Photo-Crosslinking Analysis with......ic Approach to Investigate Cytochrome P450 Cros...
- 一般注記Includes index
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- 要約等...ate-of-the-art information on ge......ntiality screenings in a wide variety......ms, i.e. screening for protein-coding genes and oth...... collection begins with chapters employing CRISPR/Cas9-b......ers also delve into other techniques for exploring essential genes, such as bioinformatics methods. Written for t......hly successful Methods in Molecular Biology series, chapters include introductions to ......on troubleshooting and avoiding known pitfall......s and Genomes: Methods and Protocols ......archers attempting to strip genetics down to it...
- 内容細目...R Screens with Inducible Cas9 CR......SPR/Cas9 Screening to Identify C......ssential Genes in Human Cell Lines Target Ident......ll Molecules Using Large-Scale C......tagenesis Scanning of Essential ......on of a CRISPR Interference Syst...... Gene Analysis in the Fungal Pat......ential Genes Using Sequential CR......ethod that Combines CRISPR and A......ssential Genes in Various Vertebrate Cell Lines New Method f......Genomics Study in Bacteria with ......rmance: CRISPR Interference Scre......d for Constructing Conditional M......ssential Genes in Cyanobacteria ...... of Transposon Insertion Sequencing in Comprehensively Identifyi...
- 件名Genes Genomics Gene editing Gènes Génomique Édition génique Gene editing. Genes. Genomics.
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- 要約等...t experiments, methods, and applicati......anopore sequencing. Chapters gui......ers through bioinformatic proced...... genome sequencing, analysis of ......lysis. Written in the format of ......hly successful Methods in Molecular Biology series, each chapter includes an introduction to t...... materials and methods, includes tips on troubleshooting and known pit......tative and cutting-edge, Nanopore Sequencing: Methods and Protocols aims to be comp...
- 内容細目...anopore sequencing Hybrid genome......and long reads in Galaxy Microbi...... genome sequencing and assembly using nanopore sequ......modification using nanopore sequ......cers Ultra-low input genome libr......anopore sequencing with droplet-...... method of eliminating the Wolbachia......t genomes from insect samples pr......ng-read sequencing A nanopore sequencing course for gr......uide to sequencing for long repe......t expansions using long DNA reads Finding rearrangements in nanopore DNA r......-genome sequencing using a nanopore se......tural variants in cancer genomes......ene analysis using long-read nanopore sequencin...
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2632 Springer protocols (Series)
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- 要約等...ewly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently appl......o other ncRNAs involved in nuclear regula......NAs, and piRNA methods applied in non-classical ......nisms. It also includes several bioinformatic and biophysical methods related to piR......stent with the increasing importance of......oughput sequencing and computational methods. Written for t......hly successful Methods in Molecular Biology series, chapters include introductions to ......on troubleshooting and avoiding known pitfall......o-date, piRNA: Methods and Protocols ......esearchers seeking to elucidate the numerous my...
- 内容細目...ects of Non-Coding RNAs on Nucle......ability Revisiting the Glass Treatment for Single Molecule An......A Function Low Input Genome-Wide......Analysis with Minimal Library Am......is of RNA-Protein Interactions for ......if Discovery Using FOREST Computational Methods for the Discov......ation of Viral Integrations Bioinformatics Appro......ches for Determining the Functiona......ents on Non-Coding RNAs Extending and Running the Mosquito ...... Resource Pipeline Preparation of Non-Overlapping Transposable ...... Annotation by Interval Tree Sta......s Approach for Intracellular Phase Separation
- 件名Small interfering RNA Small interfering RNA.
RNA modifications : methods and protocols (Methods in molecular biology ; 2298) (Springer protocols)
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- 要約等... described herein can be applied......ological and clinical questions. Beginning with a section on bioinformatics tools......collection continues with sections on detecting RNA modifications using Nanopore direct RNA sequencing, next-generation sequencing approaches, qPCR- and molecular biology-based methods, mass spectrom...... and NMR-based methods, as well as ap......hes to assess kinetics, determinants, and funct......hly successful Methods in Molecular Biology series style, chapters include introduction to t......on troubleshooting and avoiding known pitfall......Modifications: Methods and Protocols serves as an id...
- 内容細目...fication Utilizing the Gamma-Toxin Endonuclease A......lysis of Queuosine tRNA Modifications Using APB Northern ......t Assays Detecting ADP-Ribosylation in RNA Detecting Internal N7-Methylguanosine mRNA Modifica......De Novo Sequencing of RNA Mixtures Containing Both Canonica......ed Nucleosides in the Context of......to Monitor the Introduction of P...... Modifications in tRNAs with NMR...... Overview Assaying the Molecular Determinants and Kinetics of RNA Ps......lone Pseudouridine Synthases Investigating Pseudouridyla......igh-Throughput In Vitro RNA Pseu......ion and Sequencing Targeted RNA m6A Editing Using Engineered CRISPR-Cas9...
- 件名RNA editing RNA Processing, Post-Transcriptional RNA editing.
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- 要約等...chnologies and methods--combined with new gen......ools available in animal models--used in this constantly evolving field. The chapters in this book are organized into three sectio......ee explores bioinformatics and imaging analysis for ......cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfalls. Cutting-edge and comp...... Regeneration: Methods and Protocols ......esearchers looking to expand the......egeneration, aging, and regenerative medicine
- 内容細目...lation, and Staining of Murine Muscle Satell......ell Colony Forming Cell Assay as...... from Regenerating Skeletal Muscle Measuring Oxygen Consum......on Rate (ECAR) in Muscle Stem Cells Using a Seahorse An......cability for Aging Studies High ......roughput Screening of Mitochondr...... Bioenergetics in Myoblasts and ......al Progenitors in Skeletal Muscl......vated Cell Sorting and Tissue Clearing In Vitro Maturati......tem Cells from Induced Pluripote......em Cells Producing Engraftable S......Techniques for Injury, Cell Tran......gical Analysis in Skeletal Muscle Murine Models of Tenotomy-Induced Mechanical Overloading a...
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2640 Springer protocols (Series)