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- 内容細目Front Cover Series Page Methods in ENZYMOLOGY Copyright Cont...... of dihydrouridine modifications in RNA 1 Introduction 1.1 ......A function 1.2 Methods to detect dihydrouridine 2 Step-by-ste......A) selection using oligo (dT) ce......A) selection using oligo (dT) ma......ze selection using silica column......3.3 3′ End healing 3.4 RNA size ......lysis 4.1 Trimming of adapter se......plicate collapsing 4.3 UMI remov......l 4.4 Read mapping 4.5 Read end position gathering 4.6 D-site id......ion 5.2 Sequencing depth and bio......olution sequencing technology for mapping pseudouridines in mammalian mRNA......olution sequencing technology 2 Materials 2.1 P...
- 関連情報Methods in enzymology
- 掲載誌Methods in enzymology
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- 内容細目Front Cover Series Page Methods in Enzymology Copyright Cont......ructured RNA 1 Introduction 2 General methods of RT-PCR using MarathonRT 2....... Before you begin 2.2 Key resour......ls 2.4.1 Annealing RT primers to......es 2.4.2 Preparing the reverse t......ure 2.4.3 Carrying out the rever...... 2.4.4 Programming a thermal cyc......er 2.4.5 Preparing the PCR mixture 2.4.6 Carrying out the PCR a......tion 2.4.7 Examining the PCR produ......nd troubleshooting 2.8.1 Potenti......ons for optimizing the procedure...... Sanger sequencing of RNAs containing complex RNA r......ents 2 General methods of RNA Sanger sequencing usin...
- 関連情報Methods in enzymology
- 掲載誌Methods in enzymology
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- 内容細目Section 1: Protein protocols/protein precipiation: ......ction II: Protein protocols/protein pull-down methods: Coimmunopreci......tation of proteins from yeast Coupling antibody to c......alysis of protein-protein interactions by c......tion III: Protein protocols/protein purification: ......of hydrophobic interaction chrom......raphy for protein purification H......gies for recombinant proteins Salting out of proteins using ammonium sulf......recipitation Using ion exchange ......purify a recombinantly expressed protein Gel filtration......raphy) of proteins Section IV: Protein protocols/puri...... membrane proteins: Expression and purification...
- 件名Proteins--Analysis. Proteins--Purification.
- タイトル標目Methods in enzymology ; v. 541.
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- 内容細目... Northern blotting Explanatory c......tection assays In vitro transcri......Measurement of in vivo synthesis......thod for measuring mRNA decay rate in saccharomyces ......ysis for determining mRNA and ribo......me association in saccharomyces ......NA degradation intermediates Cir...... RNA radiolabeling Fluorescently labeling synthetic RNA......ntration determination RNA purif......-precipitation methods Reverse transc......dideoxy sequencing of RNA RNA st......RNA backbone using in-line probing.
- タイトル標目Methods in enzymology ; v. 530.
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- 要約等In this volume we......cols concentrating on Protein, carefully written and edited...
- 件名Proteins--Analysis. Proteins--Purification......zymologie Proteine Purification
- タイトル標目Methods in enzymology ; v. 559.
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- 内容細目...atory chapter: introducing exogenous DNA into cells Agaros...... southern blotting Purification ......igos by denaturing polyacrylamid...... Molecular cloning Rapid creatio......ammalian cell lines for regulate......ession of proteins using the Gateway® recombination cloning technology and Flp-In-REx® lines Restrictionless cloning Isolation of ......dideoxy sequencing of DNA Prepar......eration sequencing on the applie......eration sequencing Generating mammalian stable cell lines by electropo......h polyethylenimine (PEI) Site-di......r: troubleshooting PCR Explanatory chapter: qua...
- タイトル標目Methods in enzymology ; v. 529.
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- 要約等...most 50 years, Methods in Enzymology is one of the ......d publications in the field of b......0,000 chapters in the collection......or researchers in all fields of life sciences, including microbiology,...... This volume brings together a n......cols concentrating on protein, carefully wri......ed by experts, including: Pulse-chase ......o measure protein degradationLabeling a protein with fluorophores using NHS ester der......zationImmunoaffinity purification of proteinsProteolytic affinity tag cleavag......ST-tagged proteins Indispensable too......xperts to contain step-by-step p......core protocols involving protein.
- 件名Proteins--Analysis.
- タイトル標目Methods in enzymology ; v. 536.
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- タイトル標目Methods in enzymology ; v. 533.
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- 件名Enzymology.
- 一般注記Includes indexes.
- タイトル標目Methods in enzymology ; v. 582.
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- 内容細目...e prediction using the RNACompos......Adamiak ; Modeling complex RNA t...... Computational methods toward accurat......e prediction using coarse-grained and all-atom...... Andrey Krokhotin and Nikolay V.......holyan ; Improving RNA secondary...... structure mapping data / Michae......ary structures including pseudoknots b......cal graph sampling approach / Na......rmodynamics. Using reweighted pulling simulations t......tional changes in riboswitches /......modynamic and kinetic folding of riboswitch...... L. Hofacker ; Integrating molecular dyn......h chemical probing experiments using SHAPE-FIT / S......rdal Kirmizialtin, Scott P. Hennelly, Alexander...
- タイトル標目Methods in enzymology ; v. 553.
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- 件名Cell Membrane--enzymology. Membranes--enzymology. Membrane Proteins.
- タイトル標目Methods in enzymology ; v. 583.
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- 件名Cell Membrane--enzymology. Membranes--enzymology.
- タイトル標目Methods in enzymology ; v. 584.
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- ジャンル・形式用語Conference papers and proceedings.
- 件名...Congresses. Luminescent probes--......ent probes. Luminescent probes.
- タイトル標目Methods in enzymology ; v. 581.
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- 内容細目...cimen behavior in the electron b......a collection using direct detect......nkat Dandey, Clinton S. Potter a......rragher Processing of cryo-EM mo....../ Zev A. Ripstein and John L. Rubinstein Processing of structural......s cryo-EM data in RELION / Sjors H.W. Scheres Single-particle refinement and varia......ility analysis in EMAN2.1 / Stev......tory tool for single-particle cr......Grigorieff Testing the validity of single-particle ma......for model building and optimization into near-atomic ......nd Wah Chiu Refinement of atomic structures against cryo-EM Maps......tructure determination using segmented helical image reco...
- タイトル標目Methods in enzymology ; v. 579. 0076-6879
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- 内容細目... Sulfide Signaling / Peter Nagy ......urement of H2S In Vivo and In Vitro by the M......mane Method / Xinggui Shen, Gopi......de Detection Using Nucleophilic ......es / Bo Peng, Ming Xian Azide-Ba......nt Probes: Imaging Hydrogen Sulfide in Living Systems / Vivian S. Lin, Alexander R. ...... Chang Chemiluminescent Detectio......oncentration Using Methylene Blu......obenzoic Acid) Methods in Plants / Zhong......i H2S Analysis in Biological Samples Using Gas Chromatog......Sulfur Chemiluminescence Detecti......onors / Chung-Min Park, Ming Xian GYY4137,......ble, H2S-Releasing Molecule / Pe......drogen Sulfide in Parkinson's Disease Animal Mo...
- タイトル標目Methods in enzymology ; v. 554.
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- 内容細目...of metal ions binding to proteins Assess coupled protein folding and binding through tempe......nt-based screening for enzyme inhibitors using calorimetry Measuring multivalent binding interactions by i......uplex DNA-containing sugar and bas......n of cyclodextrin-mediated deter...... membrane-protein reconstitution Extending ITC to kinetics with KinlTC Measuring the kinetics of molecu......reactions Avoiding buffer interference in ITC experiment......ydrogenase ITC methods for assessing buffer/protein interactions from...... steady-state kinetics: a reacti......alysis of protein folding by differential scanning calorimetry A guide to diffe...
- タイトル標目Methods in enzymology ; v. 567.
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- 内容細目How to study intermediate filaments in atomic detail ...... properties of intermediate filament proteins Multidimensional monitoring of keratin intermediate filaments in cultured cells......body probes of intermediate filament proteins Assays for po......difications of intermediate filament proteins Immunofluores......ection of keratins - High-throughput screening for drugs that moderate intermediate filament proteins The use of Withaferin A to study intermediate fila......of cytoplasmic intermediate fila......epidermal keratins Using data mining and computati......aches to study intermediate fila......alysis of keratins and keratin-associated prote...
- 件名Intermediate filament proteins.
- タイトル標目Methods in enzymology ; v. 568.
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- 内容細目Studying Circadian rhy......hm and sleep using genetic screens in Drosophila Dissecting the mechanisms of the clock in neurospora Hig......ches for measuring circadian rhythms in cyanobacteria using bioluminescence Using circadian entrainment to find cryptic clock......adian behavior in seminatural conditions Methods to study molec......an clock Detecting KaiC phosphor......ian oscillator in vitro and in vivo The role of casein kinase I in the Drosophila......of PERIOD protein complexes of t......nd biophysical methods to analyze clo......eq and RNA-seq methods to study circa...... transcription in mammals ChIPpng away at the D...
- タイトル標目Methods in enzymology ; v. 551.
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- 内容細目Front Cover; Peptide, Protein and Enzyme Des...... Catalysts; 1. Introduction; 2. ......ugates as Protein Modification C......II) Conjugates in Organic Soluti......Metalated FKBP Inhibitor (Coughlin et al., 2014) ......II) Conjugates in Aqueous Soluti...... of an SH3 Domain and Gel-Based ......ration of Protein Modification B......Catalyzed Protein Modification; ......ion of SH3 Domain of Yes Kinase in Mammalian Cell......Vohidov, Coughlin, & Ball, 2015)......ne-Tagged Protein by Chemical Blotting3.4.1. Fluores......dified SH3 Domain; References; C......ter Two: Cell-Binding Assays for Determining the Affinity of Protein-Pr...
- タイトル標目Methods in enzymology ; v. 580.
- 著者標目Pecoraro, Vincent L.
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- 内容細目Chemokine detection using recetors immo...... and cell-cell interactions in cancer with mi......devices Generating chemokine analogs with ......l properties using phage display Methods for the recogn......AG-bound chemokines Monitoring scavenging activity of chemokine receptors Dua......tion for chemokine receptor signaling Analysis of arrestin recruitment to chemokine receptors by bioluminescence resonan......y transfer Probing biased signaling in chemokine receptors Mut......e display Studying chemokine control of ne......phil migration in vivo in a murine model of inflammatory arth......ction of chemokine/chemokine receptor complexes...
- 件名Chemokines. Chemokines--Physiological effect.
- タイトル標目Methods in enzymology ; v. 570.