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- 要約等...al killer (NK) cell research. Beginning with an assortment of methods for the isolation of NK cells and NK cell differentiatio......the volume continues with method......oxicity, viral infection, and me......, as well as clinical applications of NK cells. Written for ......hly successful Methods in Molecular Biology series, chapters include introductions to ......on troubleshooting and avoiding known pitfall......al Killer (NK) Cells: Methods and Protocols ......esearchers looking to contribute......of basic and clinical NK cell research
- 内容細目Isolation of Innate Lymphoid Cells from Murine Intestinal Lamina Propria Purif......Natural Killer Cells for Functional Analysis In Vitro Developm......e and Human NK Cells from Hematopo......tic Progenitor Cells Induction of Huma......Natural Killer Cells under Defined Conditions b......nsition from Maintenance Culture......uripotent Stem Cells Development o......odels for Studying Human NK Cells in Health and Dis...... Conjugation Using a SPAAC Coppe......10x Genomics' Single-Cell RNA-Seq Methods to Analyze the......man Primary NK Cells Using Monocle and SCENIC Analyses Methods for Isolating and Defining Single-Cell Transcriptomes...
- 件名Killer cells--Research--Methodology
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- 要約等...mentation pertaining to chemistry, biology, physiology, p......gy of CCN proteins. The chapters in this book cove......roarray; utilizing public molecu......ancreatic beta cell proliferation;......of CCNs; novel cell biological assays for measuring bone remodeling activities of CCN proteins; and the function of CCN2 in tubular epithelium cells with a focus ......nesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfalls. Cutting-edge and comp......sive, CCN Proteins: Methods and Protocols,......esearchers looking for new approaches and novic...
- 内容細目...ancreatic Beta Cell Proliferation ...... CCN Functions in Liver Regenera......fects of LIPUS-Induced CCN Proteins: Induction by LIPU......-Related Genes in Cultured Meniscus Cells and Meniscus ......y of CCN2/CTGF in Intramembranous O......ogenesis Novel Cell Biological Assays for Measuring Bone Remodeling Activities of CCN Proteins Imaging Analysis of N......s by CCN2 Protein in Neuronal Precursor Cells Derived from Human Induced Pluripotent Stem Cells Gene Expressi......ifferentiation In Vivo Angiogene......is of CCN Proteins Using the Bleomycin-Induced Model of ......on of CCN Proteins to Scleroderm......urements of Retinal Microvessel Stiffness in Mi...
- 件名Cysteine--Laboratory manuals Protein-protein interactions--Lab......y manuals Cysteine. Protein-protein interactions.
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- 要約等...d of tissue engineered vascular ....... The chapters in this book cove......s such as deriving vascular cells from monocytes and induced pluripotent stem cells; engineering vascular grafts using various bioma......rials and stem cells, stem cell-derived, or primary vascular cells; biomaterial ......vascular bioengineering technologies ......uch as 3D bioprinting; and fabricat......ocols for grafting and evaluatio......ascular grafts in animal models, vascular imaging in animals, and t......ility. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respectiv...
- 内容細目...ular Tissue Engineering Applications using Mulberry and ......erry Silk Proteins Fabrication a......n of Tissue-Engineered Vascular ......ucture Controlling Pore Size of ......by Electrospraying of Poly(Ethyl......Microparticles Injectable Hydrog......ular Tissue Engineering Rabbit Surger......s Vascular Imaging in Small Animals Using Clinical Ultrasound......Implantation using a Bilateral E......ortoiliac Preclinical Model Quantification of In Vitro Blood-Brain Barrier Permeability Subcellular Force Quan......of Endothelial Cells using Silicone Pill......s Vortices within the Cerebral Vasculature using Information Entropy
- 件名Vascular grafts Tissue engineering Blood vessel ......logy Tissue Engineering Blood Vessel ......Vaisseaux sanguins--Croissance Vaisseaux sanguins--Histologie B......wth. Tissue engineering. Vascular grafts.
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- 要約等...chnologies and methods--combined with new gen......ools available in animal models--used in this constantly evolving field. The chapters in this book are organized into three sectio......rs muscle stem cells and progenitor cells; Section Two ......or muscle stem cells and regenerat......ee explores bioinformatics and imaging analysis for muscle stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfalls. Cutting-edge and comp......al Muscle Stem Cells and Regeneration: Methods and Protocols is a valuable t...
- 内容細目...ence-Activated Cell Sorting and Tissue Clearing In Vitro Maturati......uripotent Stem Cell-Derived Myotub......al Muscle Stem Cells from Induced Pluripotent Stem Cells Sphere-Based ......uripotent Stem Cells Producing Engraftable S......uripotent Stem Cells via Teratoma ......Techniques for Injury, Cell Transplantatio......gical Analysis in Skeletal Muscle Murine Models of Tenotomy-Induced Mechanical Overloading and Tail-Suspension-Induced Mechanical Unloading Skeletal Musc......e Regeneration in Zebrafish Methods to Monitor Cir......Clock Function in Skeletal Muscle Visualizing MyoD Oscillations in Muscle Stem Cells In Vivo Mod...
- 件名Stem cells Muscle cells Muscle cells. Stem cells.
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- 要約等... plant systems biology on three speci......latory network inference and dynamic modeling using ordinary differentia......ls for at-will induction of plan......hematical modeling of plant tissue using SimuPlant or ......af; and simulating crop root systems using OpenSimRoot o......-SWMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfall......ensive and cutting-edge, Plants Systems Biology: Methods and Protocols,......biologists looking for different......approaches to finding solutions to ......ons and generating new ideas, as well as for st...
- 内容細目...SWMS How to Define the Appropria......ments When Solving Water Flow in Root System Hy......ctures? Simulating Crop Root Systems using OpenSimRoot F......ure Challenges in Plant Systems Biology Plant Systems Biology: Further Reading and Resources
- 件名Plant molecular biology Systems biology Systems Biology Biologie moléculaire végét...
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- 要約等... of up-to-date methods in the fields of ......ics. Organized into four major s......the volume examines methods utilizing model animal ......nd amphibians, methods using plants to stu......toxicity, applying the Ames assa......dy, as well as methods for environmen......ook is divided into these parts, the methods can be used ac......hly successful Methods in Molecular Biology series, chapters include introductions to ......on troubleshooting and avoiding known pitfall......icogenomics: Principles, Methods, and Applicati......uate students, inspiring more research in the vitally important field o...
- 内容細目...s elegans Germ Cell Apoptosis Following Exposure to E......onmental Contaminant Mixtures: A......Metal Mixtures in Caenorhabditis......tal Pollutants-Induced Mitochond......ial Toxicity Using Caenorhabditi......a Model System Methods to Assay the B......Toxicity Study Investigating the Joint Effects of Pe......et-B Radiation in Xenopus laevis......llutants Employing Multiple New ......eurobiological Methods to Investigate Envir......eurotoxicology in Mice Analyses ......c Modification in Environmental Pollutants-Induced Neurotoxi......s Technologies in the Research o......ture Red Blood Cell Death Practical Methods and T...
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2326 Springer protocols (Series)
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- 要約等...ree genome editing technologies,......nimal. Written in the successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfall......tative and cutting-edge, Genome Editing in Animals: Methods and Protocols,......er their study in this field
- 内容細目...Evaluation of Zinc Finger Nucleases U...... SNPD-CRISPR: Single Nucleotide ......lymorphism-Distinguishable Repre......R System Utilizing large functio......by cytoplasmic injection of CRIS......RNA Gene targeting in mouse embryonic stem cells via CRISPR/Ca......ribonucleoprotein (RNP) mediated genome editing Generation of Knock-in Mouse by Genome Editing Introduction of g......etic mutations into mice by Base......AID Genome editing in mouse and rat ......of flox mice using in vitro Cre recombination VCre/Vlox......specific recombination systems for genome engineering Efficient CRI......-Assisted Knockin of Large DNA Donors by Pronuc...
- 件名Animal genetic engineering Transgenic animals Gene editing Genetics--Tec......mal genetic engineering. Gene editing. Genetics--Technique. Transg...
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- 要約等...ate-of-the-art information on ge......ntiality screenings in a wide variety......ms, i.e. screening for protein-coding genes and oth...... collection begins with chapters employing CRISPR/Cas9-b......ers also delve into other techniques for exploring essential genes, such as bioinformatics methods. Written for t......hly successful Methods in Molecular Biology series, chapters include introductions to ......on troubleshooting and avoiding known pitfall......s and Genomes: Methods and Protocols ......archers attempting to strip genetics down to it...
- 内容細目...uripotent Stem Cells by Genome-Sca......R Screens with Inducible Cas9 CR......SPR/Cas9 Screening to Identify C......ssential Genes in Human Cell Lines Target Ident......ll Molecules Using Large-Scale C......tagenesis Scanning of Essential ......on of a CRISPR Interference Syst...... Gene Analysis in the Fungal Pat......ential Genes Using Sequential CR......ethod that Combines CRISPR and A......ssential Genes in Various Vertebrate Cell Lines New Method f......Genomics Study in Bacteria with ......rmance: CRISPR Interference Scre......d for Constructing Conditional M......ssential Genes in Cyanobacteria ...... of Transposon Insertion Sequencing in Comprehe...
- 件名Genes Genomics Gene editing Gènes Génomique Édition génique Gene editing. Genes. Genomics.
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- 要約等...e provides the increasing number of SAR...... handbook covering multidisciplinary approaches ......ogether by leading laboratories ......Topics covered include techniques in clinical and diagno......asic protocols in cell and virus cult...... as well as bioinformatics and p......ics approaches in cellular response s......on also covers methods in immunology, an......virals and vaccine development s......hly successful Methods in Molecular Biology series, chapters include the kind of detailed i......al for success in the lab. Pract......y, SARS-CoV-2: Methods and Protocols ......for scientists investigating this prevalent and...
- 内容細目...udy Virus-Host Interactions During SARS-CoV-2 Infection Human N......sal Epithelial Cells (hNECs) Gener...... by Air-Liquid Interface (ALI) C......ystem for Studying the Pathogene...... Antigens, by Single and Multipl......hemistry Measuring Neutralizing Antibodies to SARS-CoV-2 Using Lentiviral Sp...... Strategies Against SARS-CoV-2: A Systems Biology Approach Neutr......for SARS-CoV-2 Infection: Plaque......sed Drug Screening Assay for SAR......oV-2 Strengthening Biorisk Management in Research Labor......CoV-2 Research in a Biosafety Le...... Core Facility Methods of SARS-CoV-2 Inactivation
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2452 Springer protocols (Series)
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- 要約等...zed analytical methods on a wide rang......ipids. From sphingoglycolipids t......mulant present in lipopolysaccha......he book delves into how glycolip......surface of the cell membrane, whic......een organisms. In addition, glyc......important role in infections in animals and ot......nisms by targeting host cell membranes, if ......nsidered as living organisms. Wr......hly successful Methods in Molecular Biology series, chapters include introduction to t......on troubleshooting and avoiding known pitfall......, Glycolipids: Methods and Protocols ......lipid research in an effort to help researchers...
- 内容細目... and Molecular Interaction Analy......erial Lipid As Innovative Vaccine Strategy: Self-Adjuvanting Conjugate Vaccines Chemical Syn......truction of Sphingolipid Remodeled Cells by Genome Editing Mass Spectrom......eutral Glycosphingolipids Struct......he Glycolipids in Lipid Rafts Ex......Microbiota Strains NMR Analysis ......of Glycolipids in the Plasma Mem......pecific Probes in Combination with Sodi......e Replica Labeling (SDS-FRL) Bio......nalyses for Sphingolipids and It......ated Molecules in Phagosomes Single-Molecule Imaging of Ganglioside Probes in Living Cell Plasma Membran......ion of Enzymes Involved in Glycolipid Metabolis...
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2613 Springer protocols (Series)
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- 要約等...ewly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently appl......o other ncRNAs involved in nuclear regula......NAs, and piRNA methods applied in non-classical ......nisms. It also includes several bioinformatic and biophysical methods related to piR......stent with the increasing importance of......oughput sequencing and computational methods. Written for t......hly successful Methods in Molecular Biology series, chapters include introductions to ......on troubleshooting and avoiding known pitfall......o-date, piRNA: Methods and Protocols ......esearchers seeking to elucidate the numerous my...
- 内容細目...hod for Evaluating Effects of Non-Coding RNAs on Nucle......ability Revisiting the Glass Treatment for Single Molecule An......A Function Low Input Genome-Wide......Analysis with Minimal Library Am......is of RNA-Protein Interactions for ......if Discovery Using FOREST Computational Methods for the Discov......ation of Viral Integrations Bioinformatics Appro......ches for Determining the Functiona......ents on Non-Coding RNAs Extending and Running the Mosquito ...... Resource Pipeline Preparation of Non-Overlapping Transposable ...... Annotation by Interval Tree Sta......s Approach for Intracellular Phase Separation
- 件名Small interfering RNA Small interfering RNA.
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- 要約等This volume provides methods on different a...... multiplex cloning. Chapters cov......olic and signaling pathways, advanced engineering of metabolic ......organisms, and methods for the on com......bility of proteins. Written in the format of ......hly successful Methods in Molecular Biology series, each chapter includes an introduction to t...... and reagents, includes tips on troubleshooting and known pit......tative and cutting-edge, Plant Synthetic Biology: Methods and Protocols ......searchers starting to explore no......or experts willing to expand their portfolio of...
- 内容細目...plant terpenes in the photosynth......gn of multiplexing CRISPR/Cas9 c......lant genome engineering using the GoldenBra......for genetic engineering of cyanobacte......ed mutagenesis in plants Generat......robes Gene editing in green alga Chlamydomonas reinhardtii via CRI......ribonucleoproteins Coexpression ......matic cascades in bacteria using UbiGate Homo-FRET imaging to study protein-protein interaction and c......plex formation in plants Plant X......iptional units in plants AGROBES......ression system in Arabidopsis seedlings Genetically-......analysis of auxin dynamics in plant cells Exploiting the Gal4/UAS syst...
- 件名...tion Synthetic Biology Biologie synth......logy Synthetic biology
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- 要約等...at plant virus infection. Beginning with a sectio......es for identifying and studying the virus resistance gene involved in plant innate immunity, the volume continues by delving into techniques r......us resistance, methods for the analys......e of RNA silencing, as well as methods for the develo......lant viral vaccines. Written for......hly successful Methods in Molecular Biology series, chapters include introductions to ......on troubleshooting and avoiding known pitfall......ral Resistance in Plants: Methods and Protocols ......esearchers working to combat the serious threat...
- 内容細目...andidate Genes Insertion of Epitope Tag into NB-LRR Class......esistance Protein Reverse geneti......sistance signaling and the resis......ance mechanism in Arabidopsis th......sistance Signaling Pathways by Virus-Induced Gene Silencing in Nicotiana bent......istance Gene A Cell-free Replicati......cts Plant Protein-Mediated Inhibition of Virus Cell-to-Cell Movement -Far-western Screening and Biologica......f a Plant Protein Interacting with a Viral Movement Protein- Transfection ......9-mediated Editing of Genes Encoding rgs-CaM-like Proteins in Transgenic Pot......d Method to Engineer CRISPR/Cas9-Mediated Geminivirus Resistance in Plants Co...
- タイトル標目Antiviral resistance in plants (Kobayashi) Springer protocols (......ies) 1949-2448 Methods in molecular biology (Clifton, N.J.) ; v. 2028.
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- 要約等...for ES and iPS cell technology on ......uripotent stem cells, physiologica......asurements, bioinformatic analysis, gene editing technology, and cell transplantatio......udies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfall......tative and cutting-edge, Pluripotent Stem-Cell Derived Cardio...... experiments using pluripotent stem cell-derived cardiac cells
- 内容細目Making Cardiomyocyte......uripotent Stem Cells A method for ......ction of Human Induced Pluripotent Stem Cells Large-scale D......iation of Stem Cell-Derived Cardio......ocytes by Stirring-Type Suspensi...... to Dissociate Induced Pluripotent Stem Cell-derived Cardio......yte Aggregates into Single Cells Isolation of ......uripotent Stem Cells using miRNA switche......c Constructs using bio-3D Printer Fabrication......rate for Repairing Infarcted Myocard......diac Tissues Using Layer by Laye......neration of Cylindrical Engineered Cardiac T......from human iPS Cell-derived Cardiovascular Cell Lineages Protocol for Morphol...
- 件名Embryonic stem cells--Laboratory m......ratory manuals Cellules souches em......Embryonic stem cells.
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- 要約等...rice molecular biology, genetic engineering, and genome editing technologies. Dividing into three parts,...... as genetic engineering and tissue culture of rice, including efficient methods for rice trans......on, genome editing, targeted integration, and gene stacking in rice, including multiple methods utilizing CRISPR system......n via base editing, and diverse methods describing bioinformatic, molecular, and cellular analyses in rice. Written ......hly successful Methods in Molecular Biology series, chapters include introductions to ......on troubleshooting and avoiding known pitfall......Rice Genome Engineering and Gene Editing: Metho...
- 内容細目...ent genome editing in rice protoplasts using CRISPR/CAS9 construct Single transcript ......for genome editing in rice Improving a quantitative trait in rice by multigene editing with CRISPR-C......9 Rice haploid inducer developme...... by genome editing FLP-mediated ......-specific gene integration in rice Rice gene targeting by homologous recombination with posi...... start sites using TransPrise: A novel machine learning approach Single cell type specific ......ssion analysis in rice using laser capture......sis of C4 proteins in the leaf tissu......ession studies in rice Rice prot......nalyses of domains amplified in plant genomes: ...
- 件名Rice--Genetic engineering Rice--Molecul......ts Botany Microbiology Genetics Botan......Genetics. Microbiology. Rice--Genetic engineering.
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- 要約等...s applications in molecular biol......tions to determine the involvement of glycans in virus interactions. Cha......glycan and lectin microarray, pr......ation of recombinant viral domain protein, reverse genet...... and receptor binding, virus-host interactions and receptor binding, and sialidase: assays and inhibitors. Written in the successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfall......tative and cutting-edge, Glycovirology: Methods and Protocols aims to be an e...
- 内容細目Comprehensive cellular glycan profiling of glycoproteins and glycosphingolipids by glycoblotting and BEP methods High-performan......matography mapping method for th......the molecular, cellular, and tissu......c acids on the cell surface using flow cytometry Lectin Staining to Detect Human and Avian influenza Virus Receptors in the Airway of ......lycan Arrays Using Glycopeptides......s Glycan profiling of viral glycoproteins with the lectin microarray Pre......ation of recombinant PA endonuclease domain protein of influenza A virus......ation for glycobiology research Receptor-binding specificity of influenza viruses.-Functional an...
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2556 Springer protocols (Series)
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- 要約等.... The chapters in this book ae organized into four parts: ......synthesis, combinational library......esis and screening, macrocycle c...... Two describes methods for the creati......bsequent screening against biological targets of interest. Part Th......rocycle-target interactions, and Part Four introduces unique......tions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfalls. Cutting-edge and comp......e Macrocycles: Methods and Protocols ......esearchers looking to learn more......ut this developing field
- 内容細目Three Methods for Peptide Cy......tivation Three Methods for the Soluti......rocycles On-Resin Peptide Cyclization Using the 3-4-Amino-(Methylamino)Benzoic Acid (MeDbz) Linker Scalable and Efficient in planta Biosynt......Sunflower Trypsin Inhibitor-1 (SFTI......Formation Protein Catalyzed Capt...... Antigen Targeting Preparation of Bacterial Cell-Surface Displa......athogenic Protein Misfolding and Aggregation by Integrating SICLOPPS Tech......roughput Screening in Bacteria In Vitro Selectio...... Display Assessing the Cellular Uptake, En......yclic Peptides In silico Analysi......acrocycle-Protein Interactions Binding Characte...
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2371 Springer protocols (Series)
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- 要約等... the latest engineering methods of mammalian cells that are useful for controlling the performance of engineered mammalian cells for future cell-based therapeu......tter understanding of complex bi....... The chapters in this book are organized into five parts. ...... One described methods to engineer mammalian cells to sense biol......cally relevant inputs, such as cell contacts and soluble proteins. Part Two loo......chniques to engineer mammalian cells to sense artificial inputs, such as l......e provides cutting-edge CRISPR-Cas-based methods to carry out h......xed genome editing and spatiotem......led genome editing. Part Four discusses ways to...
- 内容細目... Gene Switches in Mammalian Cells Multiplexed Genome Engineering with Cas12a H......SPR Genome Editing Outcomes in Mammalian Cells Optical Contr...... of Genome Editing by Photoactiv......ontrol of Protein Localization and Mammalian Cell Signaling by SLIPT Engineering Hydrogel Production in Mammalian Cells to Synthetica......trollable Protein Aggregates in Cells Intracellular Unnatural ......der-Free Human Induced Pluripotent Stem Cell Culture using a DNA Aptamer......ovo Gene Targeting via In Utero Electrop......ically Guided Single Cell Virus Stamping
- 件名Mammals--Genetic engineering--Laboratory m......uals Synthetic biology--Laboratory manuals Bioengineering--Laboratory manuals Bioengineering. Synthetic biology.
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- 要約等...t-Learn (DBTL) into synthetic biology research. Chap......l systems, setting up of an auto......gn, RNA sequencing, microfluidics -using bacterial cell free extracts,......live mammalian cells, computationa......arts can be engineered in mammalian cells to sense, and......espond to, and intracellular signals in general. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfall......tative and cutting-edge, Synthet......Gene Circuits: Methods and Protocols ......essful results in the further study of this vit...
- 内容細目Qualitative Modeling, Analysis and......ene Circuits Using Models To (Re......th SYNBADm Setting up an Automat......d Biomanufacturing Laboratory Co......ter-aided Planning for the Verif......cts Characterizing Genetic Parts and Devices using RNA Sequencing Steady-State Cell-Free Gene Expr......ene Expression in Mammalian Cells Optimal Exper...... and Synthetic Biology Using Amigo A Cyber...... Prediction of cellular burden wit......de for Quantifying Retroactivity in Gene Networks Engineering Sensors for G......sion Burden Engineering Protein-based Parts fo......enetic Devices in Mammalian Cells
- 件名Synthetic biology--Laboratory ma......orks Synthetic Biology--methods Synthetic biology.
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- 要約等...te-of-the- art methods on computer-ai......eaders through information on an......ructures, modeling antibody stru......tibody-antigen interactions, and......ed antibody affinity maturation ......eyond. Written in the format of ......hly successful Methods in Molecular Biology series, each chapter includes an introduction to t...... and reagents, includes tips on troubleshooting and known pit......tative and cutting-edge, Compute......nd experts looking to expand their knowledge
- 内容細目...ure analyses using IMGTª: 30 years of immunoinformatics Struc......tion of CDR-H3 in Single-Domain VHH Antibodies......tational modelling of Antibody and T Cell receptor (CDR3......simulation for investigating antigen-antibody interaction Molecular Dynamics Methods for Antibody Design Probing antibody dyna......Estimation of Single Domain Antibodies Using Molecular Dyn......ulations Assessing and engineering antibody stability using experimental ...... computational methods In Silico Predict......ethod for Protein Asparagine Deamidation S......lar Modelers B-cell epitope predictions using computational methods Computational Epitope Predict...
- 件名Immunoinformatics Immunoglobulins--Computer-aided design Immunoinformatics.