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- 件名case-oriented analysis ki-shou-ten-ketsu informed consent cancer patients shared decision making
- 著者標目Taichi Hatta Keiichi Narita Kazuhiro Yanagihara Hiroshi Ishiguro Toshinori Murayama Masayuki Yokode
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- 要約等...ewly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently appl......o other ncRNAs involved in nuclear regula......NAs, and piRNA methods applied in non-classical ......nisms. It also includes several bioinformatic and biophysical methods related to piR......stent with the increasing importance of......oughput sequencing and computational methods. Written for t......hly successful Methods in Molecular Biol......ries, chapters include introductions to ......on troubleshooting and avoiding known pitfall......o-date, piRNA: Methods and Protocols ......esearchers seeking to elucidate the numerous my...
- 内容細目Functional Analysis of Individual piRNAs in Aedes aegypti ...... and Embryos Using Antisense Oli......ated Genome Engineering in Aedes aegypti ......rected DNA Elimination for Tetra......ian PIWI-piRNA Interaction Analysis Using Immunoprecipi......d piRNA-Sequencing Isolation and Processing of Bovine Oocytes for S......all RNA Sequencing 3D Imaging and In Situ Hybridiza......ion for Uncovering the Functions of MicroRNA in Rice Anther Cloning, Sequencing, and Linkage Analysis o......es for Elucidating piRNA Pathway...... Somatic Cell Lines Using the piggyBac ......t RNA FISH Combined with Immunof......Ribonucleoproteins in the Drosophila Germline C...
- 件名Small interfering RNA Small interfering RNA.
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- 要約等...ate-of-the-art information on ge......ntiality screenings in a wide variety......ms, i.e. screening for protein-coding genes and oth...... collection begins with chapters employing CRISPR/Cas9-b......ers also delve into other techniques for exploring essential genes, such as bioinformatics methods. Written for t......hly successful Methods in Molecular Biol......ries, chapters include introductions to ......on troubleshooting and avoiding known pitfall......s and Genomes: Methods and Protocols ......archers attempting to strip genetics down to it...
- 内容細目...R Screens with Inducible Cas9 CR......SPR/Cas9 Screening to Identify C......ssential Genes in Human Cell Lines Target Ident......ll Molecules Using Large-Scale C......tagenesis Scanning of Essential ......on of a CRISPR Interference Syst...... Gene Analysis in the Fungal Pat......ential Genes Using Sequential CR......ethod that Combines CRISPR and A......ssential Genes in Various Vertebrate Cell Lines New Method f......Genomics Study in Bacteria with ......rmance: CRISPR Interference Scre......d for Constructing Conditional M......ssential Genes in Cyanobacteria ...... of Transposon Insertion Sequencing in Comprehensively Identifyi...
- 件名Genes Genomics Gene editing Gènes Génomique Édition génique Gene editing. Genes. Genomics.
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- 要約等...ree genome editing technologies,......nimal. Written in the successful Methods in Molecular Biol......rmat, chapters include introductions to ......on troubleshooting and avoiding known pitfall......tative and cutting-edge, Genome Editing in Animals: Methods and Protocols,......er their study in this field
- 内容細目...Evaluation of Zinc Finger Nucleases U...... SNPD-CRISPR: Single Nucleotide ......lymorphism-Distinguishable Repre......R System Utilizing large functio......by cytoplasmic injection of CRIS......RNA Gene targeting in mouse embryoni......ribonucleoprotein (RNP) mediated genome editing Generation of Knock-in Mouse by Genome Editing Introduction of g......etic mutations into mice by Base......AID Genome editing in mouse and rat ......of flox mice using in vitro Cre recombination VCre/Vlox......specific recombination systems for genome engineering Efficient CRI......-Assisted Knockin of Large DNA D......ronuclear Microinjection During S-phase in Mous...
- 件名Animal genetic engineering Transgenic animals Gene editing Genetics--Tec......mal genetic engineering. Gene editing. Genetics--Technique. Transg...
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- 要約等...mentation pertaining to chemistry,......gy of CCN proteins. The chapters in this book cove......roarray; utilizing public molecu......says for measuring bone remodeling activities of CCN proteins; and the function of CCN2 in tubular epithe......nesis. Written in the highly successful Methods in Molecular Biol......rmat, chapters include introductions to ......on troubleshooting and avoiding known pitfalls. Cutting-edge and comp......sive, CCN Proteins: Methods and Protocols,......esearchers looking for new appro......hers just starting out in the field of CCN research
- 内容細目CCN Proteins - Cellular Co......Factors: Expanding their Reperto......uorescence Imaging for the Evalu......CN Family Proteins- Imaging of Molecular Interaction between CCN Protein and Its Binding Partners: An In Situ Proximity......ation Assay of Interaction Betwe......CCN2 and Rab14 in Chondrocytes W......is of CCN Proteins and Fragments in Exosomes, Vesi......of CCN Factors in/on Exosomes Ev...... the Molecular Interaction between CCN Protein and Its Binding Partners: A Solid-Phase Binding Assay and Sur......cols for Screening Peptides Binding to CCN Family Proteins and their Ext......ransfection, Spinfection, Exofection, and Lucif...
- 件名Cysteine--Laboratory manuals Protein-protein interactions--Lab......y manuals Cysteine. Protein-protein interactions.
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- 内容細目Front Cover Series Page Methods in ENZYMOLOGY Cop...... of dihydrouridine modifications in RNA 1 Introduction 1.1 ......A function 1.2 Methods to detect dihydrouridine 2 Step-by-ste......A) selection using oligo (dT) ce......A) selection using oligo (dT) ma......ze selection using silica column......3.3 3′ End healing 3.4 RNA size ......lysis 4.1 Trimming of adapter se......plicate collapsing 4.3 UMI remov......l 4.4 Read mapping 4.5 Read end position gathering 4.6 D-site id......ion 5.2 Sequencing depth and bio......olution sequencing technology for mapping pseudouridines in mammalian mRNA......olution sequencing technology 2 Materials 2.1 P...
- 関連情報Methods in enzymology
- 掲載誌Methods in enzymology
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- 要約等...t experiments, methods, and applicati......anopore sequencing. Chapters gui......ers through bioinformatic proced...... genome sequencing, analysis of ...... transcriptome analysis. Written in the format of ......hly successful Methods in Molecular Biol......, each chapter includes an introduction to t...... materials and methods, includes tips on troubleshooting and known pit......tative and cutting-edge, Nanopore Sequencing: Methods and Protocols aims to be comp...
- 内容細目...anopore sequencing Hybrid genome......and long reads in Galaxy Microbi...... genome sequencing and assembly using nanopore sequ......modification using nanopore sequ......cers Ultra-low input genome libr......anopore sequencing with droplet-...... method of eliminating the Wolbachia......t genomes from insect samples pr......ng-read sequencing A nanopore sequencing course for gr......uide to sequencing for long repe......t expansions using long DNA reads Finding rearrangements in nanopore DNA r......-genome sequencing using a nanopore se......tural variants in cancer genomes......ene analysis using long-read nanopore sequencin...
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2632 Springer protocols (Series)
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- 要約等...l research. Beginning with an assortment of methods for the isolat......the volume continues with method......oxicity, viral infection, and me......, as well as clinical applicatio......hly successful Methods in Molecular Biol......ries, chapters include introductions to ......on troubleshooting and avoiding known pitfall......er (NK) Cells: Methods and Protocols ......esearchers looking to contribute......of basic and clinical NK cell research
- 内容細目Isolation of Innate Lymphoid Cells from Murine Intestinal Lamina Propria Purif......ional Analysis In Vitro Developm......ogenitor Cells Induction of Huma......Cells under Defined Conditions b......nsition from Maintenance Culture......odels for Studying Human NK Cells in Health and Dis...... Conjugation Using a SPAAC Coppe......10x Genomics' Single-Cell RNA-Seq Methods to Analyze the......ary NK Cells Using Monocle and SCENIC Analyses Methods for Isolating and Defining Single-Cell Transc......ic Color-Barcoding for Fate Mapping of Single Innate Lymphocytes Quantifying Human Natural......gration by Imaging and Image Analysis Tracking ...
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2463 Springer protocols (Series)
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- 要約等...chnologies and methods--combined with new gen......ools available in animal models--used in this constantly evolving field. The chapters in this book are organized into three sectio......ee explores bioinformatics and imaging analysis for ......cells. Written in the highly successful Methods in Molecular Biol......rmat, chapters include introductions to ......on troubleshooting and avoiding known pitfalls. Cutting-edge and comp...... Regeneration: Methods and Protocols ......esearchers looking to expand the......egeneration, aging, and regenerative medicine
- 内容細目...lation, and Staining of Murine Muscle Satell......ell Colony Forming Cell Assay as...... from Regenerating Skeletal Muscle Measuring Oxygen Consum......on Rate (ECAR) in Muscle Stem Cells Using a Seahorse An......cability for Aging Studies High ......roughput Screening of Mitochondr...... Bioenergetics in Myoblasts and ......al Progenitors in Skeletal Muscl......vated Cell Sorting and Tissue Clearing In Vitro Maturati......tem Cells from Induced Pluripote......em Cells Producing Engraftable S......Techniques for Injury, Cell Tran......gical Analysis in Skeletal Muscle Murine Models of Tenotomy-Induced Mechanical Overloading a...
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2640 Springer protocols (Series)
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- 要約等... of up-to-date methods in the fields of ......ics. Organized into four major s......the volume examines methods utilizing model animal ......nd amphibians, methods using plants to stu......toxicity, applying the Ames assa......dy, as well as methods for environmental chemical analysis. Although this ......ook is divided into these parts, the methods can be used ac......hly successful Methods in Molecular Biol......ries, chapters include introductions to ......on troubleshooting and avoiding known pitfall......icogenomics: Principles, Methods, and Applicati......uate students, inspiring more research in the vitally important field o...
- 内容細目...poptosis Following Exposure to E......onmental Contaminant Mixtures: A......Metal Mixtures in Caenorhabditis......tal Pollutants-Induced Mitochond......ial Toxicity Using Caenorhabditi......a Model System Methods to Assay the B......Toxicity Study Investigating the Joint Effects of Pe......et-B Radiation in Xenopus laevis......llutants Employing Multiple New ......eurobiological Methods to Investigate Envir......eurotoxicology in Mice Analyses ......c Modification in Environmental Pollutants-Induced Neurotoxi......s Technologies in the Research o......eath Practical Methods and Technologies in Environmental Epidemiology In...
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2326 Springer protocols (Series)
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- 要約等"This Handbook of Research Methods in Careers serves......esearchers use in career scholarship. Presenting detailed over......ce of research in the discipline. Chapters sho......se diverse and interdisciplinary approaches to studying careers acros......ive, and mixed methods. Providing an in-depth illustra......of established methods and current trends in careers resear......his Handbook brings together top international aut...... choices. Offering cutting-edge methods from established and emerging experts, this......is crucial reading for scholars ......currently studying or wish to st...... be useful for institutions coordinating large ...
- 内容細目...er, John McMackin, Szu-Hsin Wu, Jose Aldo ......er decision making / Gregory Hen......y Yip 6. Designing and studying mentoring programs: Rev....... Quantitative methods 7. Text mining in career studies: Generating insights from uns......l tell: Conducting longitudinal research on ......ocial networks in contemporary c......ultilevel modeling for careers r......tive and mixed methods 11. Engaging grounded theo......careers: Attending to the relati......reenberg 12. Using in-depth interviews in careers resear......identities and institutions: The......antitative examination of metaph......l language use in career-life preparedness / Al...
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- 要約等.... The chapters in this book cove......ted DNA sequencing; target enrichment sequencing via probe cap......transformation methods; prediction of long non-coding RNAs and seco......; genome-wide mining of disease re......gs; genome editing, and bioactiv......hosphoproteome analysis. Written in the highly successful Methods in Molecular Biol......rmat, chapters include introductions to ......on troubleshooting and avoiding known pitfalls. Cutting-edge and informative, Legume Genomics: Methods and Protocols ......ert researcher involved in various molecular aspects of ...
- 内容細目... Genome Sequencing Updates on Ge......omic Resources in Chickpea for C......Construction using an Iterative Mapping and Assembly Approach Methods and Tools for ...... Genome Sequencing, Assembly and......tream Analysis Methods of Gene Expression Profiling to Understand......n and Response in Legume Crops M......ulphite Sequencing Data Analysis......tion Detection in Legumes Method......Example Genotyping for Species I......y Assessment using Double-Digest......ted DNA Sequencing (ddRAD-seq) F......omic Selection in Crop Breeding Methods for Target Enrichment Sequencing via Probe Capture in Legumes Developing Transposable Element Marker ...
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2107 Springer protocols (Series)
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- 内容細目Front Cover Series Page Methods in Enzymology Cop......ructured RNA 1 Introduction 2 General methods of RT-PCR using MarathonRT 2....... Before you begin 2.2 Key resour......ls 2.4.1 Annealing RT primers to......es 2.4.2 Preparing the reverse t......ure 2.4.3 Carrying out the rever...... 2.4.4 Programming a thermal cyc......er 2.4.5 Preparing the PCR mixture 2.4.6 Carrying out the PCR a......tion 2.4.7 Examining the PCR produ......nd troubleshooting 2.8.1 Potenti......ons for optimizing the procedure...... Sanger sequencing of RNAs containing complex RNA r......ents 2 General methods of RNA Sanger sequencing using MarathonRT 2.2 Key res...
- 関連情報Methods in enzymology
- 掲載誌Methods in enzymology
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- 要約等...s applications in molecular biol......tions to determine the involvement of glycans in virus interactions. Cha......glycan and lectin microarray, pr......ation of recombinant viral domain protein, reverse genet...... and receptor binding, virus-host interactions and receptor binding, and sialidase: assays and inhibitors. Written in the successful Methods in Molecular Biol......rmat, chapters include introductions to ......on troubleshooting and avoiding known pitfall......tative and cutting-edge, Glycovirology: Methods and Protocols aims to be an e...
- 内容細目...r glycan profiling of glycoproteins and glycosphingolipids by glycoblotting and BEP methods High-performan......matography mapping method for th......cell surface using flow cytometry Lectin Staining to Detect Human and Avian influenza Virus Receptors in the Airway of ......lycan Arrays Using Glycopeptides......s Glycan profiling of viral glycoproteins with the lectin microarray Pre......ation of recombinant PA endonuclease domain protein of influenza A virus......arch Receptor-binding specificity of influenza viruses...... of sulphatide in influenza A virus infection and rep......nt motility of influenza A particles by biolaye...
- タイトル標目Methods in molecular biology (Clifton, N.J.) ; v. 2556 Springer protocols (Series)
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- 要約等...ers on longitudinal studies, ran......d decisions making, and a new section on changing health-relate....... The chapters in this book are organized into six parts: P......focuses on framing clinical research q......tions and choosing a suitable de......that may occur in clinical research; ......ociated with doing conducting research on h......ysis that pertain to evaluation of risk in longitudinal studies; ass......ent of therapy in controlled tri...... Five presents methods used in various compon......ed decision-making; and Part Six highlights interventions focused on changing health-relate......viors. Written in the highly successful Methods...
- 内容細目...rials 6: Determining the Sample Si......nd Power for Clinical Trials and......ls 7: On Contamination and Estimating the Actual Tr......ative Research in Clinical Epidemiolo......ed Decision Making 1: Critical A......ed Decision Making 2: Systematic......ed Decision Making 3: Health Tec......ed Decision Making 4: Clinical Practice Guidelines Evidence-Based Decision Making 5: Knowledge ......ed Decision Making 6: Administrative Datab......rvice Research Methods in Evidence-Based Decision Making 7: Health Economics in Clinical Research Methods in Evidence-Based Decision Making 8: A Primer f......searchers Changing Health-Related Behaviors 1: ...
- 件名... Epidemiologic Methods Research Design Evidence-Base...
RNA modifications : methods and protocols (Methods in molecular biology ; 2298) (Springer protocols)
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- 要約等... described herein can be applied......ological and clinical questions. Beginning with a section on bioinformatics tools......collection continues with sections on detecting RNA modifications using Nanopore direct RNA sequencing, next-generation sequencing approaches, q...... biology-based methods, mass spectrom...... and NMR-based methods, as well as ap......hes to assess kinetics, determinants, and funct......hly successful Methods in Molecular Biol......tyle, chapters include introduction to t......on troubleshooting and avoiding known pitfall......Modifications: Methods and Protocols ...... for those working directly in the fields of ep...
- 内容細目...fication Utilizing the Gamma-Toxin Endonuclease A......lysis of Queuosine tRNA Modifications Using APB Northern ......t Assays Detecting ADP-Ribosylation in RNA Detecting Internal N7-Methylguanosine mRNA Modifica......De Novo Sequencing of RNA Mixtures Containing Both Canonica......ed Nucleosides in the Context of......to Monitor the Introduction of P...... Modifications in tRNAs with NMR...... Overview Assaying the Molecular Determinants and Kinetics of RNA Ps......lone Pseudouridine Synthases Investigating Pseudouridyla......igh-Throughput In Vitro RNA Pseu......ion and Sequencing Targeted RNA m6A Editing Using Engineered CRISPR-Cas9...
- 件名RNA editing RNA Processing, Post-Transcriptional RNA editing.
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- 要約等...gy, genetic engineering, and genome editing technologies. Dividing into three parts,...... as genetic engineering and tissue culture of rice, including efficient methods for rice trans......on, genome editing, targeted integration, and gene stacking in rice, including multiple methods utilizing CRISPR system......n via base editing, and diverse methods describing bioinformatic, molec......lular analyses in rice. Written ......hly successful Methods in Molecular Biol......ries, chapters include introductions to ......on troubleshooting and avoiding known pitfall......Rice Genome Engineering and Gene Editing: Methods and Protocols serve...
- 内容細目...genome editing in rice protoplasts using CRISPR/CAS9 construct Single transcript ......for genome editing in rice Improving a quantitative trait in rice by multigene editing with CRISPR-C......9 Rice haploid inducer developme...... by genome editing FLP-mediated ......-specific gene integration in rice Rice gene targeting by homologous recombination with posi...... start sites using TransPrise: A novel machine learning approach Single cell type s......ssion analysis in rice using laser capture......sis of C4 proteins in the leaf tissu......ession studies in rice Rice prot......nalyses of domains amplified in plant genomes: ...
- 件名Rice--Genetic engineering Rice--Molecul......ce--Genetic engineering.
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- 要約等...d carrier protein dependent synthases, including polyketide sy......mistry, and bioinformatics. Written in the format of ......hly successful Methods in Molecular Biol......, each chapter includes an introduction to t...... materials and methods, includes tips on troubleshooting and known pit......tative and cutting-edge, Non-Rib......nthesis and Engineering: Methods and Protocols ......ims to feature methods that will be b...... and those wanting to adopt new methodologies into their research
- 内容細目The Assembly-line Enzymology of...... Synthetases Using NMR Titration to Study Protein-Protein Interactions in AcpP Dependent......roteomic Profiling of Adenylation Domain Functions in Gramicidin S-producing Nonribosomal ......de Synthetases In Vitro Biochemi......sed Macrocyclizing Thioesterase Domains from Non-Ribo......s by a Penicillin-binding Protein-type Thioester......A Carrier Protein Domain for Recognitio......Phosphopantetheinyl Transferases to Enable Inhibitor Screening Unraveling Structural Information of Multi-Domain Nonribosomal P......nthetases by Using Photo-Crosslinking Analysis with......ic Approach to Investigate Cytochrome P450 Cros...
- 一般注記Includes index
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- 要約等...cts, molecular methods for identifying, and current trends in designing non-natural n......egulators, and in-vitro studies. Written in the format of ......hly successful Methods in Molecular Biol......, each chapter includes an introduction to t...... and reagents, includes tips on troubleshooting and known pit......tative and cutting-edge, Engineering Natural Produ...... Biosynthesis: Methods and Protocols ......nd experts looking to expand the......ttribution 4.0 International License via link.springer.com
- 内容細目A bioinformatics workflow for investigating fungal biosyn...... gene clusters Investigating Fungal Biosyn......tic Pathways Using Heterologous ......erologous host Investigating Fungal Biosyn......tic Pathways Using Heterologous ......erologous Host Investigating Fungal Biosyn......tic Pathways Using Heterologous ......hetic Pathways in Aspergillus nidulans using episomal vect......ted Genetic Engineering via Agrobacte......transformation in Fusarium solani Investigating Fungal Biosyn......tic Pathways Using Pichia pastor......ionary genome mining for the discovery and engineering of natural pr......t biosynthesis Inducing Global Expression of Ac...
- 件名... Biomedical engineering--Laboratory m...... Biomedical engineering Natural products--Biotechnol...
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- 要約等...at plant virus infection. Beginning with a sectio......es for identifying and studying the virus resistance gene involved in plant innate immunity, the volume continues by delving into techniques r......us resistance, methods for the analys......e of RNA silencing, as well as methods for the develo......lant viral vaccines. Written for......hly successful Methods in Molecular Biol......ries, chapters include introductions to ......on troubleshooting and avoiding known pitfall......ral Resistance in Plants: Methods and Protocols ......esearchers working to combat the serious threat...
- 内容細目...andidate Genes Insertion of Epitope Tag into NB-LRR Class......esistance Protein Reverse geneti......sistance signaling and the resis......ance mechanism in Arabidopsis th......sistance Signaling Pathways by Virus-Induced Gene Silencing in Nicotiana bent......cts Plant Protein-Mediated Inhibition of Vir......-western Screening and Biologica......f a Plant Protein Interacting with a Viral Movement Protein- Transfection ......9-mediated Editing of Genes Encoding rgs-CaM-like Proteins in Transgenic Pot......d Method to Engineer CRISPR/Cas9-Mediated Geminivirus Resistance in Plants Computa......mall RNA Profiling in Virus-Infected Plants A S...
- タイトル標目Antiviral resistance in plants (Kobayashi) Springer protocols (......ies) 1949-2448 Methods in molecular biology (Clifton, N...