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- 要約等This book provides state-of-the-art information on gene ......a wide variety of organisms, i.e. screening for protein-coding genes and other genomic eleme......ith a focus on the two techniques......revolutionized the field, the collection beg......so delve into other techniques for exploring esse......, such as bioinformatics methods. Written for the highly successful Methods in Molecular B......troductions to their respective topics, lists of the necessary mate......s and Genomes: Methods and Protocols ......an ideal guide for researchers attempting to str...
- 内容細目...e Essentiality of Human Pluripot......Identification of Small Molecules Using Large-Sc......nesis Scanning of Essential Gene......and Generation of a CRISPR Interference System for Genetic Repres......ne Analysis in the Fungal Pathoge......Identification of Essential Gene......onal Knockouts for Essential Gene......nes New Method for Genome-Scale F......ional Genomics Study in Bacteria with Superior Performance: CRISPR I......R-Based Method for Constructing C......onal Mutations of Essential Gene......teria A Method of Transposon Ins......Genes Required for Growth in Human Serum The Use of Tn-Seq and the FiTnEss Analysis to Define th...
- 関連情報1850-9999 Methods in molecular biology Springer...
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- 要約等This volume looks at the latest technologies and methods--combined with......volving field. The chapters in th...... animal models for muscle stem ce...... explores bioinformatics and imaging analysis for muscle stem ce......ls. Written in the highly successful Methods in Molecular Biology series format, chapters i......troductions to their respective topics, lists of the necessary mate...... Regeneration: Methods and Protocols ...... valuable tool for all researcher......king to expand their knowledge on skeletal muscl...
- 内容細目..., and Staining of Murine Muscle ......, and Analysis of Zebrafish Myofibres and Assoc......tal Myogenesis The Satellite Cell Colony Forming Cell Assay......al Co-Cultures of Macrophages wi...... Applicability for Aging Studies ......hput Screening of Mitochondrial ......Myotubes State of the Art Procedures for the Isolation and ......aracterization of Mesoangioblasts Analyses of Mesenchymal Pr......tro Maturation of Human Pluripot......ifferentiation of Human Fetal Mu......ased Expansion of Myogenic Proge......s via Teratoma Formation Techniques for Injury, Cell T...... Murine Models of Tenotomy-Induc......n in Zebrafish Methods to Monitor Circadian Clock Fu...
- タイトル標目Methods in molecular biology (Clifton...
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- 内容細目Front Cover Series Page Methods in Enzymology ......-to-end RT-PCR of long RNA and h......-to-end RT-PCR of long RNA and h......tion 2 General methods of RT-PCR using M......rathonRT 2.1 Before you begin 2.2.......4.2 Preparing the reverse transc......3 Carrying out the reverse transc...... Programming a thermal cycler 2.4.5 Preparing the PCR mixture 2.......6 Carrying out the PCR amplificat.......4.7 Examining the PCR products b......tial solutions for optimizing the procedure 3 Su......ger sequencing of RNAs containin......ents 2 General methods of RNA Sanger seq......GE) to analyze the RNA sequence 2......erative cycles of directed evolution 2 Library ...
- 関連情報Methods in enzymology
- 掲載誌Methods in enzymology
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- 要約等...lume discusses the latest techniq......tocols used in the field that wer......not covered in the previous edition. The chapters in th......d phenotypic profiling, and Part......co integration of functional gen......ta. Written in the highly successful Methods in Molecular Biology series format, chapters i......troductions to their respective topics, lists of the necessary mate......onal Genomics: Methods and Protocols,......uable resource for all researcher......ing more about the evolving field of yeast
- 内容細目...in Yeast using the 10x Genomics C...... Genome-Wide Profiling of Transcription ......nking Analysis of cDNAs (CRAC) Protocol for Detecting RNA-......eep Sequencing of Nascent Transc......ect Sequencing of RNA and RNA Mo......Identification of Taxonomically ......equencing Data FORK-seq: Single Molecule Profiling of DNA Replication CUT&RUN Profiling of the Budding Yeast ...... albicans and Other Yeasts Purification of Ribosome-Nasce......-Chain Complex for Ribosome Profiling and Selec......ive Ribosome Profiling Single-St......s Spectrometry of Macromolecular Complexes in the Yeast S. cerev......n and Dynamics of Protein Complexes Measured by...
- 関連情報Methods in molecular biology Springer...
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- 要約等...nal approaches for the analysis of many aspects vital to the understanding of membrane prote......ve guidance on the selection and use of methods for over-expressio......s to determine the structures of membrane prote......rimental steps for specific membr......al notes allow the protocols to b......stems. Written for the highly successful Methods in Molecular B......apters include the kind of practical information and impl......nction Studies of Membrane Prote......an ideal guide for biologists, bi......striving to further understand these essential proteins and their many biological roles
- 内容細目Expression and Purification of Human Mitochon......Reconstitution of Detergent-Solu...... and Nanodiscs for Functional and......aracterization of GPCR-G Protein Complex Formation Electrop......cal Approaches for the Study of Ion Channel Function Isothermal Titration Calorimetry of Membrane Proteins Atomic Force Microscopy R......in Activity at the Single Molecul...... Determination of Membrane Prote......rystallography Studying Membrane Pr......rticle Cryo-EM of Membrane Prote......tallization in the New Era of Electron Cryo-......scopic Studies of Ion Channels i......Exemplified by the Voltage Depend......el Preparation of a Deuterated Membrane Protein...
- タイトル標目Methods in molecular biology (Clifton...
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- 要約等...h protocols to study the structure and dynamics of bacterial and ...... and assembly, the book continues......riven motility of bacteria, arch......riven motility of archaea, type ......ching motility of bacteria, as w......iding motility of bacteria and other unique motili......stems. Written for the highly successful Methods in Molecular B......troductions to their respective topics, lists of the necessary mate......al Motility is the ideal reference for researchers wo......his vital area of microbiology
- 内容細目Purification of the Transmembrane ......hannel Complex of the Salmonella Fla......n-Close Motion of a Flagellar Ex......e-Cell Imaging of the Assembly and E......tion Processes of the Bacterial Flag......Image Analysis of the Bacterial Flag......otope Labeling of FliG for Studying Structural ......o Measurements of the Ion Channel Activity of the Transmembrane ......tor Complex in the Bacterial Flag......r Purification of the Na+-Driven Pom......y Purification of Na+-Driven Mot......h-Speed Atomic Force Microscopy H......Rotation Assay of the Bacterial Flag......scence Imaging of Magnetosome Organelle for Magnetotaxis Motility Swarmin...
- タイトル標目Methods in molecular biology (Clifton...
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- 内容細目...aphic Analysis of AcrB Using Des......aphic Analysis of Drug and Inhib......ding Structure of RND-Type Multi......aphic Analysis of MATE-Type Mult......aphic Analysis of the CusBA Heavy-Me......Efflux Complex of Escherichia co......- Purification of AcrAB-TolC Mul......ug Efflux Pump for Cryo-EM Analys......py Approach to Study the Structure, Ori...... and Mechanism of the Multidrug Expo......mrE Generation of Conformation-Specific......body Fragments for Crystallization of the Multidrug Resi......aracterization of Multi-Componen......Linked Trimers of RND (Resistanc......ransporters to Study Their Mechanism of Action: Es...
- タイトル標目Methods in molecular biology (Clifton...
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- 要約等The purpose of this book is to convey to the worldwide scie......ific community the rapid and enth......astic progress of state-of-the-art quantum ch......upercomputers. The usefulness of quantum chemis......deals with all of these topics. Frontiers of Quantum Chemis......ely related to the symposium of the same name held......November 2015. The book's contrib......ed speakers at the symposium but also many other distinguished......rom wide areas of quantum chemistry around the world.
- 内容細目...ingle Particle Theories Circular ......ctroscopy with the SAC-CI Methodo......gy: A ChiraSac Study Frontiers of Coupled Cluste......tical Response Theory Response Theory and Molecul......nse Properties of Periodic Mater......antum Chemical Methods for Predicting and......d p-conjugated Molecules Embedding Methods in Quantum Che......ry Calculation of Vibrational Spectra of Large Molecules from their Fragments Describing Molecules in Motion by Q......ntum Many-Body Methods Relativistic T......ity-Functional Theory for Molecular Prop......ity Functional Theory Toward a Qu......al Description of Molecular Properties and Chem...
- 件名Quantum chemistry. SCIENCE--Chemistry--Physical & Theoretical. Computer Appl. in Li...
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- 内容細目Studying Circadian r......ila Dissecting the mechanisms of the clock in neuro......ive approaches for measuring circ......based analysis of circadian beha......netic analysis of Drosophila cir......ral conditions Methods to study molecular mechanisms of the neurospora cir......lation rhythms of the cyanobacterial......ro and in vivo The role of casein kinase I in the Drosophila cir......n and analysis of PERIOD protein complexes of the mammalian circ......est practicies for fluorescence microscopy of the cyanobacterial......nd biophysical methods to analyze clo......Identification of small-molecule modulators of the circadian clock ChIP-seq ...
- タイトル標目Methods in enzymology ; v. 551.
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- 内容細目Overview : assays for studying integrin-de......-time analysis of integrin-dependent transendothelial migration ......itial motility of leukocytes / Z......sfer method to study integrin funct......ing in studies of the binding betwee...... I domains and their ligands / Th......binding assays for integrin LFA-1......ctural biology of integrins / Gu......d purification of integrin I dom......d IgSF ligands for crystallograph......scopic imaging of integrin / Ken...... NMR method to study protein-protei......ingle-molecule methods to study cell adhesion molecules / Joonil Seog ......w : imaging in the study of integrins / Christop...
- 件名Cell adhesion molecules--Laboratory manuals. Cell Adhesion Molecules Integrins
- タイトル標目Methods in molecular biology (Clifton...
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- 要約等Handbook of Modern Coating......aracterization Methods focuses on new aspects of surface engine......coatings, from their fabrication methods to properties ......olume explores the detection of surface featur......Subjects cover the production of new, advanced coatings and the characteristics of abrasion, corrosion, fatigue ...
- 内容細目Front Cover Handbook of Modern Coating...... Contents List of contributors About the editors Prefac...... 1 Application of the scanning vibra......e technique to the characterization of modern coating......3.1 Strategies of corrosion cont......3.5 Techniques for assessing coat......egradation 1.4 The scanning vibra......echnique 1.4.1 The principle 1.4.......Quantitative information 1.4.6 Li......7 Main sources of artifacts and .......5 Application of the scanning vibra......ings anodic to the substrate (Cas......gs cathodic to the substrate (Cas...... 9) 1.6 Review of published work using the scanning vibrating electrode ...
- 並列タイトル等（連結）Advanced characterization methods
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- 内容細目... Wave function theories and elect......onic structure methods: quantum chemi...... from atoms to molecules Density functional theory: from conce......onic structure methods at work New keys for old keywords: ......and properties The modeling in mo......usters Add on. the bondon: a new theory of electron effective coupling a...
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- 内容細目... Spectroscopy: The Virtual Multif......ntum Chemistry for Studying Electronic ......y and Dynamics of Complex Molecu......Young Min Rhee Theoretical and Ex......r Spectroscopy of the Far-Ultraviole......antum Approach of IR Line Shapes of Carboxylic Acids Using the Linear Response Theory / Paul Blai......Adina Velcescu Theoretical Calcul......Strong Tool in the Investigation of Strong Intramo......ral Simulation for Flexible Molecules in Solution wi......ation Analysis of Matrix-Isolati......yuki Akai Role of Quantum Chemic......anical Studies of Vibrational Spectra of Hydrogen-Bonde......cal Simulation of Near-Infrared Spectra: Applic...
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- 内容細目...hta User guide for the LORE1 insertio......put sequencing for Tnt1 flanking ...... recovery / Xiaofei Cheng, Nick ......Wen Generation of doubled haploid lines for QTL mapping / ......d Julin N. Maloof Assessing dist...... and variation of genome-wide DN...... chromosome conformation capture ...... Genome-wide profiling of histone modifi......ranscriptome profiling in Arabid......tion protocols for protein abunda......sphoproteome profiling of plant tissues ......ot phenotyping of Arabidopsis th......le phenotyping of root traits in the model legume L......nfocal imaging of Arabidopsis thaliana roots for simultaneous quantification o...
- 件名...ant. Genomics--methods. Plants--genetics.
- 別の記録形式Table of contents
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- 要約等...rvational test of the planet formation theory based on the theoretical study of the water snowline......ch water takes the form of ice, in the protoplanetary disks - the place where planets are formed. Since the water snowline......ught to divide the regions of rocky and gas-giant planet formation, the location of the snowline is essential for the planet formation process. The book proposes ......thod to locate the snowlines usin......c observations of water vapor li...... calculations. The author obtained the water vapor distribution in the disks using the chemical react...... interactions. The simulated transition lines of...
- 内容細目...uri Disk 2.2.3 The Case of a Candidate Or......ion Line 2.2.4 The Case of a Ortho-H216O ......ne that Probes the Hot Surface Layer 2.2.5 The Case of a Ortho-H216O ......ne that Probes the Cold Water Res.......3.1 Influence of Model Assumpti......al Density and the Assumption of LTE 2.3.3 Requirement for the Observations 2......ng Studies II. The Case of the Herbig Ae Star 3.1 Methods 3.2 Results 3.2.1 The H2O Gas and Ic......ibutions 3.2.2 The Overview of Ortho-H216O Li...... Ae Disk 3.2.3 The Case of Candidate Orth......on Lines 3.2.4 The Case of the Less Suited Or......on Lines 3.2.5 The Candidate Orth......e Fluxes 3.2.6 The Radial Distributions of Norma...
- 件名... Solar system: the Sun & planets. Astronomy, spa...
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- 内容細目...ng Determining the architecture of a protein-DNA ......tructures, and the ICM molsoft program In ce......ule approaches for the characterization of riboswitch fol......anisms Probing of nascent ribosw......tional studies of DNA-protein in......identification of genome-wide tr...... amplification of cDNA ends (5'-RACE) Analysis of DNA supercoili......identification of DNA-binding pr......ion (ChIP-exo) The cruciform DNA mobility ......say: A tool to study proteins that ......and validation of spacer sequences for CRISPR-Cas9 ge......eria Detection of short-range DN...... chromosome conformation capture ......Global mapping of open chromatin regulatory ele...
- 件名... Footprinting--methods. DNA-Binding Proteins--chemis...
- 別の記録形式Table of contents
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- 要約等...gies Discusses the role of proteolytic si......cancer, among other disease types Features methods and protocols supporting further study of proteolytic si......nal leaders in the field"--
- 内容細目...ations Cancer Other pathological ......ses Cysteine cathepsin inhibitors and the autophagic pro......nes Chemokines The roles of proteases in sepsis The roles of proteases in C......tic processing of laminin and the role of cryptides in t......GSR, short arm of beta1 chain) I......KVAV, long arm of alpha1 chain) ......RT, LG4 domain of alpha1 chain) ......ne proteases Cathepsins Exogenous......al cancer and other diseases Prot......ases: A family of proteinases involved in the balance betwee......7: A potential therapeutic biomar......erent tools to study the mechanism of regulation in ...... regulation at the substrate level ADAM17 regula...
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- 要約等...a large amount of biological sys......ers understand the results of systems immuno......ntroduced, and the systematic gen......ology and bioinformatics in their work. It is ......everal members of biomedical fie...... to illustrate the solution proce......s to represent the development procedure of systematic imm......n a very clear format Contains 10......ers understand the related biological networks, their correspondin......ork biomarkers for therapeutic drug design
- 内容細目...3. Identifying the gene regulatory network of systems inflam......on pathways in the vascular endothelial inflammato...... 5. Prediction of infection-asso......infection case study 6. Global screening of potential Candida albicans biofilm-related tra......Identification of infection- and......action network for Candida albica......tional modules for pathogenic and......on Process 10. The role of inflammation a...... biomarkers in the pathomechanisms of early cardioem......ve Immunity in the Infection Proc......ork biomarkers of pathogen-host ......e immunity 13. The coordination of defensive and offensive molecular mechanisms i...
- 件名Immunology--Mathematical models ......mune system--Mathematical models Infection--Mathematical models ...... Immunology--Mathematical models Immunology--Mathematical models. Infection--Mathematical models.
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- 内容細目The three-dimensional structure of proteins Chemical catalysis The basic equations of enzyme kinetic...... and magnitude of individual rate constants The pH dependence of enzyme catalysis Practical methods for kinetics and e......bria Detection of intermediates ......tereochemistry of enzymatic reac......inhibitors" Conformational change......tors, and work Forces between molecules, and binding e......ementarity and the use of binding energy......g Case studies of enzyme structu......ility Kinetics of protein folding Folding pathw...
- 件名...ins--Mechanism of action. Protein Conformation. Enzymes--chemistry. Ca...
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- 内容細目...aracterization of Protein Molecules Prepared by To......al Chemical Synthesis 1.1 Introdu......cal Protein Synthesis 1.3 Comment......aracterization of Synthetic Protein Molecules 1.3.1 Homogene.......4 Limitations of SPPS 1.3.5 Fol......low Peptide Synthesis 2.1 Introdu......low Peptide Synthesis 2.2.2.1 Key......2.2.2.2 Design of First-generati......aracterization of First-generati......low Peptide Synthesis 2.2.3.1 Key......2.2.3.2 Design of Second-generat......zation and Use of Second-generat......low Peptide Synthesis 2.2.4.1 Key......2.2.4.2 Design of Third-generati......aracterization of Third-generation AFPS 2.2.4.4...
- 件名Proteins--Synthesis Proteins--Synthesis.
- 一般注記4.3 Optimization of Peptide Hydrazide-based Nativ...