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- 件名cell surface display Pichia pastoris lipase
- 一般注記...属: 神戸大・工・応化 Affiliation: Grad. Sch. Sci. Tech., Kobe Univ. Affiliation: Dept. Chem. Sci. Eng., Kobe Univ. レポート・講演番号: 2I15-3
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- 要約等<p>Advancements in synthetic biology have facilitated the microbial production of valuable plant metabolites. However, constructing complete biosynthetic pathways within a single host organism remains challenging. To solve this problem, modu......ulture systems involving host organisms with partial pathways hav......We focused on <i>Escherichia coli</i>, a general host for metabolite production, and <i>Pichia pastoris</i> (<i>Komagataella phaffii</i>), a novel synthetic biology host due to its high expression of biosynthetic enzymes. Previously, we repor......co-culture of <i>E. coli</i> cells, which produce reticuline (an impo...
- 件名co-culture system benzylisoquinoline alkaloid synthetic biology <i>Pichia pastoris</i> <i>Escherichia coli</i>
- 参照De novo resveratrol production through modular engineering of an Escherichia coli–Saccharomyces cerevisiae co-culture D......ment of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products Biosynthesis of catharanthine in engineered Pichia pastoris Construction of an <scp>l</scp>-Tyrosine Chassis in <i>Pichia pastoris</i> Enhances Aromatic Secondary Metabolite Production from Glycerol A bacterial platform for fermentative production of plant alkaloids Establishment of a co-......lture system using Escherichia coli and Pichia pastoris (Komagataella phaffii) for valuable alkaloid produ...
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- 要約等<jats:title>Abstract</jats:title> ...... <jats:p>The industrial yeast Pichia pastoris has been harnessed extensively for production of proteins, and it is attracting attention as a chassis cell factory for production of chemicals. However, ......lack of synthetic biology tools makes it challenging in rewiring P. pastoris metabolism. We here extensively engineered the recombination machinery by establishing a CRISPR-Cas9 based genome editing platform, which improved the homologous recombination (HR) efficiency by more than 54 times, in particular, enhanced the simultaneously assembly of multiple fragments by 13.5 times. W...
- 件名Genetics
- 参照One-Step <i>In Vivo</i> Assembly of Multiple DNA Fragments and Genomic Integration in <i>Komagataella phaffii</i>
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- 要約等<jats:title>ABSTRACT</jats:title> ......jats:p>Dysfunction or capacity shortage of the endoplasmic reticulum (ER) is cumulatively called ER ...... unfolded protein response (UPR). In various yeast species, the ER-loca......smembrane protein Ire1 is activated upon ER s......erforms the splicing reaction of HAC1 mRNA......ture form of which is translated into a transcription factor protein that is responsible for the transcriptome change on......ssessed the splicing of HAC1 mRNA in Pichia pastoris (Komagataella phaffii) cells. We found that, inconsistent with previous reports by ......NA was substantially, but partially, spliced e...
- 件名Applied Microbiology and Biotechnology General Medicine Microbiology
- 参照Fast-Growing Saccharomyces cerevisiae Cells with a Constitutive Unfolded Protein Response and Their Potential for Lipidic Molecule Production
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- 要約等<jats:title>Abstract</jats:title> ......jats:p>Fructooligosaccharides (FOSs)—fructose-based oligosaccharides—are typical prebiotics with health-promoting effects in humans and animals. The trisaccharide 1-kestotriose is the most attractive inulin-type FOS. We previously reported a recombinant sucrose:su......hedonorus arundinaceus (Sa) that efficiently converts sucrose into 1-kestotriose. In this study, Pichia pastoris PGFT6x-308 constitutively expressing nine copies of the Sa1-SST gene displayed fructos......transferase activity in undisrupted biomass (49.8 U/m......t (120.7 U/ml) in fed-batch fermentation (72 h...
- 件名Applied Microbiology and Biotechnology Biotechnology Bioengineering
- 参照Enzymatic and structural characterization of β-fructofuranosidase from the h......ybee gut bacterium Frischella perrara
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- 要約等<jats:title>Abstract</jats:title> ...... <jats:p>Besides bakers’ yea......he methylotrophic yeast Komagataella phaffii (also known as Pichia pastoris) has been developed into the most po......for the production of heterologous proteins. Strong prom......s, stable genetic constructs and a growing collection of freely available strains, tools and p......have boosted this development e...... thorough genetic and cell biological characterization. This review provides an overview of state-of-......tools and techniques for working with P. pastoris, as well as guidelines for the production of recombinant proteins wit...
- 件名Molecular Biology Biochemistry
- 参照Phosphoregulation of the transcription factor Mxr1 plays a crucial role in the concentration‐regulated methanol induction in <i>Komagataella phaffii</i>
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- 要約等...arbohydrate-Active enZymes (CAZymes) associated with plant cell wall metabolism is important for elucidating the developmental mechanisms of plants a......also for the utilization of plants as a biomass resource.......e use of recombinant proteins is common in this context, but ......ologous expression of plant proteins is particularly difficult, in part because ......e of many cysteine residues promotes denaturation, aggregation and/or protein misfolding. In this study, we eva......of methylotrophic yeast <i>Pichia pastoris</i> as expression hosts for expansin from peach (<i>Prunus persica</i> (L.) Batsch, <i>Pp</i>EXP1),...
- 件名<i>Pichia pastoris</i> heterologous expression expansin cysteine CAZymes
- 著者標目Matsuyama Kaori Sunagawa Naoki Igarashi Kiyohiko
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- DOI10.1021/acssynbio.2c00047
- 件名Biochemistry, Genetics and Molecular Biology (miscellaneous) Biomedical Engineering General Medicine
- 参照Enhanced Co-culture System Using <i>Escherichia coli</i> and <i>Pichia pastoris</i> (<i>Komagataella phaffii</i>) for Improved Microbial Production of Valuable Plant Alkaloids
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- 要約等<jats:title>Abstract</jats:title><jats:p>One of the most important branches of genetic engineering is the expression of recombinant proteins using biological expression systems. Nowadays, different expression systems are ......for the production of recombinant proteins including bacteria, yeasts, mold......s, plants, and insects. Yeast expression systems such as <jats:italic>Saccharomyces cerevisiae</jats:italic> (<jats:italic>S. cerevisiae</jats:italic>) and <jats:italic>Pichia pastoris</jats:italic> (<jats:italic>P. pastoris</jats:italic>) are more popular. <jats:italic>P. pastoris</jats:italic> expression system is one of...
- 件名Cell Biology Clinical Biochemistry Physiology
- 参照Two-step purification of tag-free norovirus-like particles from silkworm larvae (Bombyx mori) Physiology of Methylotrophs Living in the Phyllosphere
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- 要約等In the yeast Sac......haromyces cerevisiae, terminator sequences not only terminate transcription but also affect expression levels of the protein-encoded upstream of the terminator. The non-conventional yeast Pichia pastoris (syn. Komagataella phaffii) has frequentl......orm for metabolic engineering but knowledge regarding P. pastoris terminators is limited. To explore terminator sequences available to tune protein expression levels in P. pastoris, we created a 'terminator catalog' by testing 72 sequences, including terminators from S. cerevisiae or P. pastoris and synthetic terminators. Altoget......d that the terminators have a tunable range of...
- 件名Genetics
- 参照One-Step <i>In Vivo</i> Assembly of Multiple DNA Fragments and Genomic Integration in <i>Komagataella phaffii</i> SIN-like pathway kinases regulate the end of mitosis in the methylotrophic yeast <i>Ogataea polymorpha</i>
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- 件名Applied Microbiology and Biotechnology General Medicine Biotechnology
- 著者標目Ji-Yeon Kang Hong-Yeol Choi Dong-Il Kim Ohsuk Kwon Doo-Byoung Oh
- 出版者（掲載誌）Korean Society for Microbiology and Biotechnology
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- 一般注記MUSIC:Partitur Italian words; also printed as text with German translation
- 関連情報In dulci jubilo : musik zu weihnachtszeit
- 掲載誌In dulci jubilo : musik zu weihnachtszeit
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- 要約等<jats:title>Abstract</jats:title><jats:p><ja......>l</jats:sc>‐amino acid oxidases (LAAOs) a......catalyze the oxidative deamination of <jats:sc>l</jats:sc>‐amino acids to the corresponding α‐keto acids, ammonia, and hydrogen peroxide. Here, we sh......the overexpression, purification, and the characterization of LAAO4 fro......e fungus <jats:italic>Hebeloma cylindrosporum</jats:italic> in the yeast <jats:italic>Pichia pastoris</jats:italic> with a 9His‐tag and compare this with the recently characterized 6His‐<jats:italic>hc</jats:italic>LAAO4 expressed in <jats:italic>E</jats:italic>.<jats:italic> coli</jats:italic>. The...
- 件名Microbiology
- 参照Reconstruction of Hyper‐The...... Ancestral L‐Amino Acid Oxidase to Perform Deracemization to D‐Amino Acids Antimicrobial properties of L-amino acid oxidase: biochemical features and biomedical applications
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- 要約等<jats:title>Abstract</jats:title><jats:p>Recent studies have reveale......hat some bacteria can inhabit plant seeds, and they are likely founders of the bacterial community in the rhizosphere of or inside plants at th......mental stage. Given that the seedling establishment is a critical fitness component......eedy plant species, the effects......f seed endophytic bacteria (SEB) on the seedling performance are of particular interest in weed ecology.......e, we characterized the SEB in natural populations of <jats:italic>Capsella bursa</jats:italic>‐<jats:italic>pastoris</jats:italic>, a model species of weed ecology. The compos...
- 件名Nature and Landscape Conservation Ecology Ecology, Evolution, Behavior and Systematics
- 参照Investigating plant–microbe interactions within the root
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- 要約等<jats:title>Abstract</jats:title><jats:p>Angiotensinogen (AGT), a ......ated plasma noninhibitory serpin, serves as a ......ecursor for angiotensin peptides which regulate blo......e balance. AGT is specifically cleaved by renin to produce angiotensin‐I, the first product of the angiotensin‐processing cascade. Ovine angiotensinogen (oAGT) is considered an effective substrate for human renin and consequently finds application in clinical renin assays. In this study, oAGT was cloned into the genome of <jats:italic>Pichia pastoris</jats:italic> and expresse......l of alcohol oxidase (AOX1) promoter for high‐level production. Compared ...
- 件名Biotechnology
- 参照Boosting Auto-Induction of Recombinant Proteins in Escherichia coli with Glucose and Lactose Additives
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- 要約等<jats:title>ABSTRACT</jats:title> ......the ectomycorrhizal fungus Tricholoma matsuta......(TmGLA) was purified 33.2-fold to homogeneity as a single monomeric glycoprotein with a molecular ......f 63.9 kDa. Maximum activity was observed.......0. The enzyme is active down to 50°C and in the pH range ......f 4.0–6.0, and its activity is strongly inhibited by Ag+. It degrades α-1,......nd α-1,6-glycosidic linkages in various polysaccharides. Its gene (TmGlu1) was cloned using information from the enzyme’s internal amino acid sequences and...... The deduced amino acid sequence show......lear homology with those of GH family 15 prote...
- 件名Organic Chemistry Molecular Biology Applied Microbiology and Biotechnology General Medicine Biochemistry Analytical Chemistry Biotechnology
- 参照Purification and characterization of a glycoside hydrolase family 5 endoglucanase from Tricholoma matsuta......arley based solid-state medium
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- 要約等<jats:title>Abstract</jats:title> ......ts:p>A hydroxynitrile lyase (HNL) from the millipede Chamberlinius hualienensis has high potential for industrial use in the synthesis of cyanohydrins. However, obtaining sufficient amounts of millipedes is difficult, and the production of the Chamberlinius hualienensis HNL (ChuaHNL) in E. coli has not been v...... Therefore, we investigated the conditions required for high-yield heterologous production of this enzyme using Pichia pastoris. When we employed P. pastoris to express His-ChuaHNL, the yield was very lo......re). Hence, we investigated the effec......uaHNL codon optimization and the co-production...
- 件名Applied Microbiology and Biotechnology Biotechnology Bioengineering
- 参照Discovery and Structural Analysis to Improve the Enantioselectivity of Hydroxynitrile Lyase from <i>Parafontaria laminata</i> Millipedes for (<i>R</i>)-2-Chloromandelonitrile Synthesis <i>R</i>‐hydroxynitrile lyase from the cyanogenic millipede, <i>Chamberlinius hualienensis</i>—A new entry to the carrier protein family Lipocalines Structural characterization of Linum usitatissimum hydroxynitrile lyase: A new cyanohydrin decomposition mechanism involving a cyano-zinc complex
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- 要約等<jats:title>Abstract</jats:title><jats:sec><......s are well‐studied standard tools for controlling gene expression. Acquiring control over......natural regulation of promoters is important for metabolic engineering and synthetic biology applications. In the commonly used protein production host <jats:italic>Komagataella phaffii</jats:italic> (<jats:italic>Pichia pastoris</jats:italic>), methanol‐inducible promoters a...... because of their tight regulation and exceptional strength. Yet, induction with toxic and flammable......l can be a considerable safety risk and cannot be applied in many existing fermentation plants. Her...
- 件名Applied Microbiology and Biotechnology Bioengineering Biotechnology
- 参照Engineering the expression system for <i>Komagataella phaffii (Pichia pastoris)</i>: an attempt t......ol-free expression system
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- 要約等<jats:title>ABSTRACT</jats:title> ......p>The construction of a methanol-free expression system of Ko......agataella phaffii (Pichia pastoris) was attempted by engineering a strong methanol-inducible DAS1 promoter using Citrobacter braakii phytase production as a model case. Constitutive expression of KpTRM1, f......erly PRM1—a positive transcription regulator for methanol-utilization (MUT) genes of K. phaffii,was demonstrat......oduce phytase without addition of methanol, especially when a DAS......er. Another positive regulator, Mxr1p, did not have the ......S1 promoter, while it was more effective than KpTrmp1 on the AOX1 pr...
- 件名Applied Microbiology and Biotechnology General Medicine Microbiology
- 参照Yeast Methylotrophy: Metabolism, Gene Regulation and Peroxisome Homeostasis Mit1 Transcription Factor Mediates Methanol Signaling and Regulate...... the Alcohol Oxidase 1 (AOX1) Promoter in Pichia pastoris <i>Pichia pastoris</i> 14‐3‐3 regulates transcriptional activity of the methanol inducible transcription factor Mxr1 by direct interaction Transcriptional Regulation in <i>Saccharomyces cerevisiae</i>: Transcription Factor Regulation and Function, Mechanisms of Initiation, and Roles of Activators and Coactivators Molecular characterization of <i>Candida boidinii MIG1</i> and its role in the regulation of methanol‐inducible gene ...
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- 要約等<jats:p>With nearly three......lopment, <jats:italic>Pichia pastoris</jats:italic> (<jats:italic>P</jats:italic>. <jats:italic>pastoris</jats:italic>) has become ......werful eukaryotic protein expression system for the expression of thousands of proteins both on a laboratory and industrial scale. In addition, it has also been extensively used as a ......for the production of a variety of chemicals. This review summarizes the bottlenecks and solutions in achieving high‐level secretory protein expression with <jats:italic>P. pastoris</jats:italic> and then outlines its applications on chemical production with an emphasis on its rol...
- 件名Molecular Medicine Applied Microbiology and Biotechnology General Medicine
- 参照One-Step <i>In Vivo</i> Assembly of Multiple DNA Fragments and Genomic Integration in <i>Komagataella phaffii</i>