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- 要約等...r identifying, and current trends......n heterologous expression techniques, gene disruption, mo......ay regulators, and in-vitro studi......sary materials and reagents, incl......roubleshooting and known pitfalls, and step-by-step, ...... Authoritative and cutting-edge, ......hesis: Methods and Protocols aims......to be a useful and practical guid......ew researchers and experts looking to expand their knowledge. Chapter 13 i...
- 内容細目...l biosynthetic gene clusters Inves......g Heterologous Gene Expression - Aspergillus ......g Heterologous Gene Expression: Aspergillus n......g Heterologous Gene Expression: Fusarium sp. ......t Heterologous Expression of Fungal Bios......ctors Targeted Genetic Engineering...... the discovery and engineering of......nducing Global Expression of Actinobacte......l Biosynthetic Gene Clusters Engin......l Biosynthetic Genes and Elucidating Function Understanding and Manipulating A......y Heterologous Expression in Streptomyces Heterologous expression, purification, and characterizati......e acyl carrier proteins Cyanobacterial Genome Sequen...
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- 要約等...est techniques and protocols used......tomic analyses and Part Two cover......NA replication and protein/DNA interactio......tion dynamics, protein complexes, and proteomics. Pa......otypic screens and phenotypic profiling, and Part Five expl......ssary material and reagents, step......ory protocols, and tips on troubleshooting and avoiding known....... Cutting edge and practical, Yea......omics: Methods and Protocols, Second Edition is ...
- 内容細目... Detecting RNA-Protein Interactions and Transcription ......uencing of RNA and RNA Modificati......Reconstruction and Gene Discovery usin......ool to Explore Gene-Regulatory Networks in Candida albicans and Other Yeasts Purification of Ribosome-Na......some Profiling and Selective Ribo......-Step Affinity Purification (ssAP) and Mass Spectrome......ae Composition and Dynamics of Protein Complexes Meas......al Scanning of Protein-Protein Interaction St......st: From bSLIM and Proteome Disco......ignificance of Protein Quantification......hogenic Yeast Candida glabrata High Throughput Gene Mutagenesis Screening using Base Editi...
- 件名Saccharomyces cerevisiae--Genetics Molecular genetics--Technique......ory technology Genetics--Technique......s Yeast fungi--Genetics
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- 要約等...e latest tools and techniques inv......er on genomics and the various tools and applications i......s such as next-generation sequenci......ods, protocol, and expected resul...... with diagrams and graphs. All pr......n a very lucid and precise way, t......ders to follow and replicate them
- 内容細目Chapter 1: Conventional and Basic genomic ...... Mitochondrial Genetics Chapter 4:......ary Study-Phylogenetic tree Chapte......Chapter 12: Epigenetics and DNA Methylatio......apter 14: Next Generation sequenci......ressing cloned genes for protein production, purification and analysis-Bioph......apter 20: Transgenesis and Biopharming Ch......E) Chapter 22: Gene Therapy Chapte......cs Chapter 24: Gene Editing Chapter 25: Introduct...
- 関連情報Springer protocols handbooks
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- 要約等...e challenging, and at times dange......DNA isolation, protein isolation, and lipid isolatio...... of ribosomes, and continues with......sary materials and reagents, step......ory protocols, and tips on troubleshooting and avoiding known...... Authoritative and up-to-date, My......teria research and also for those who have worke...
- 内容細目...ria Extraction and Separation of Mycobacterial Proteins Lipid and Lipoarabinomannan Isolation and Characterization Purification of Hibernating and Active C- Ribo......el Single-Cell and High Throughpu...... Microfluidics and Time-Lapse Mic......asuring Efflux and Permeability i......f Mycobacteria and Mycobacteria-I......f Mycobacteria Gene Switching and Essentiality T......Recombineering and Its Use for Ma......s, Insertions, and Fusions in Myc......) for Targeted Gene Silencing in M......ng Fluorescent Proteins to Understand Mycobacterium ......Identification and Characterizati......s Experimental and Computational Workflow for RN...
- 件名...Mycobacterium--genetics Bacterial Proteins--genetics DNA, Bacterial--genetics Mycobacterium--growth & d...
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- 内容細目pt. A. Gene expression and protein purification pt. B. Cell biology and physiology pt. C. Protein-protein interactions and genomics.
- 件名遺伝子工学. 蛋白質. 生化学的遺伝学. Gene fusion. Proteins -- Analysis. Chimeric Proteins -- analysis. Gene expression.
- 一般注記Includes bibliographical references and indexes.
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- 要約等...(<I>Rurm1</I>) gene was cloned and transformed in......ssed the RURM1 protein as a glutathio......e (GST)-fusion protein by cultivating......ained a single protein of 12 kDa. This protein was identified as the RURM1 protein by western blot analysis.
- 件名...e (GST)-fusion protein Oryza sativa recombinant protein ubiquitin-like protein
- 参照Ubiquitin and ubiquitin-like proteins as multifunct......RNA Eukaryotic proteins expressed in ......ombin cleavage and purification procedure of fusion proteins with glutathi......tion pathway A Protein Conjugation Sy...... slender glume gene in rice (Oryza...... L.) Ubiquitin and its kin: how close are the fa...
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- 内容細目...erg Strategies and considerations for protein purifications / S. Linn Use...... in planning a protein purification / R.R. Burgess Preparing a purification summary table ......rs: principles and practice / V.S. Stoll and J.S. Blanchard...... / T.K. Harris and M.M. Keshwani ......uantitation of protein / J.E. Noble and M.J. Bailey Concentration of proteins and removal of sol......s, J.K. Romero and M. Westoby Maintaining protein stability / M....... a recombinant protein / W.H. Brondyk......f heterologous proteins / S. Zerbs, A.M. Frank and F.R. Collart Expression in the yeast P......us-insect cell expression systems / D.L. Jarvis Recombi...
- 件名Proteins -- Purification. Proteins -- isolation & purification.
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- 件名Arabinogalactan-proteins Hydroxyproline
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- 内容細目Protein antigen expression in escherichia......, S.K. Backues and S.Y. Bednarek Expression of epitope-tagged proteins in plants / T. Furuichi Expression of epitope-tagged proteins in arabidopsi......sts / Y.H. Cho and S.D. Yoo Transient expression of epitope-tagged proteins in mammalian ......ers, J. Lowery and E. Sztul Production and purification of polyclonal ......a, M. Mukumoto and K. Miyatake Production and purification of monoclonal ......a, M. Mukumoto and K. Miyatake Pr......gold particles and conjugation to protein A, IgG, F(ab')₂, and streptavidin /......s / T. Osafune and S.D. Schwartzb......y of cryofixed and freeze-substituted plant tiss...
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- 要約等...tides: Methods and Protocols, a s......of established and emerging metho......for optimizing and improving cell......dy conjugates, and nanoparticles.......ells, assaying gene knockdown, sel......st target site and synthesis of v......sary materials and reagents, step......ble protocols, and notes on troubleshooting and avoiding known...... Authoritative and easily accessi......tides: Methods and Protocols serv...... professionals and novices pursui...... this exciting and pioneering field.
- 内容細目...ames W. Gaynor and Richard Cossti......gy for ex vivo and in vivo oligon......des / Samir El Andaloussi ... [ e...... Liu, Yi Wang, and Donald J. Hnat......soud M. Toloue and Lance P. Ford ...... for efficacy: gene target IL-12Rb......aura L. Hauck, and Dan V. Mourich......e pathways / Liande Li and Yi Liu Quantif......iRNAs in vitro and in vivo / Angie Cheng, Alexander V. Vlassov, and Susan Magdalen......ion conditions and analysis of si......san Magdaleno, and Alexander V. Vlassov s......A knockdown of gene expression in endothelial......mily Dennstedt and Brad Bryan Usi......autz-Peterson, and Patrick J. Skelly Performing ...
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- 内容細目...onal profiling and molecular clon......abeled RNA for gene expression measurements u......z, Liping Nie, and Ebenezer N. Ya...... to study mRNA expression and distribution i......Takunori Satoh and Donna M. Fekete Genetic fate-mappin....../ Jun Chul Kim and Susan M. Dymec......cal use of Cre and loxP technolog......ch / Yiling Yu and Jian Zuo Helios gene gun-mediated t......ation-mediated gene transfer to th...... / John V. Brigande ... [et al.] ......, Pascal Senn, and Stefan Heller ......ng-Sheng Chang and D. Bradley Wel......equence typing and pulsed field g......than C. Thomas and Melinda M. Pet......the middle ear and upper respiratory tract mucos...
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- 内容細目...sequence tags, and trace / Ju You...... Ji Yeon Park, and Bin Tian Bioin......ranscriptional gene regulation : t......UTR Collection and other informat......th E. Regulski and Ronald R. Brea......rap : targeted purification of RNA-specifi......ify regulatory proteins and RNAs / Dale L. Beach and Jack D. Keene ...... : RNA-binding protein immunoprecipit......r RNA aptamers-protein interaction / ......Maria Minunni, and Marco Mascini ......roach to study proteins that activate......human cells / Sandra L. Clement and Jens Lykke-Andersen RNA analy......a 4-thiouracil and uracil phospho......-dependent RNA purification : use in identifying and stud...
- 件名Gene Expression Regulation -- ......atory Manuals. Genetic regulation -- Laboratory m...
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- 内容細目Biopharmaceutical proteins / John R. Birch and Yemi Onakunle Expression of antibody fr......erichia coli / Andrew G. Popplewe......of therapeutic proteins in Escherichi......ong Hyun Choi, and Sang Jun Lee Expression of recombinant LTB protein in marine vibr......60 / Wang Yili and Si Lusheng Heterologous gene expression in yeast / Lee....... O'Callaghan, and Mick F. Tuite Pharmaceutical proteins from methylot......n ... [et al.] Expression of human papil......in baculovirus expression systems / Zhen......n, Si Lusheng, and Wang Yili Larg......cale transient expression of therapeutic proteins in mammalian ......Martin Jordan, and Florian M. Wurm Site-specific...
- 件名Protein drugs -- Laboratory manuals. Proteins -- therapeutic use. Proteins -- chemical synthesis. Proteins -- isolation & purification.
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- 内容細目...adhesion / Alexandre Chigaev and Larry A. Sklar...... Weitz-Schmidt and Stephanie Chre......lial migration and integrin-indep...... / Ziv Shulman and Ronen Alon Lentiviral gene transfer metho......aliya Banerjee and Motomu Shimaok......grin I domains and their ligands / Thomas Voru......n Cell-free ligand-binding assays...... Fu, Wei Wang, and Bing-Hao Luo Protein expression and purification of integrin I domains and IgSF ligands for crystallo...... Hongmin Zhang and Jia-huai Wang ......ethod to study protein-protein interactions /......ritaka Nishida and Ichio Shimada ......-cell motility and stop signals / Andrew J. Wiem...
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- 内容細目1. Analysis of gene function using...... microarrays / Andrew P. Capaldi ...... data analysis and visualization ......g / Maki Inada and Jeffrey A. Ple......ois, Wei Zheng and Michael Snyder......t / Oliver J. Rando 6. Genome-wid......II. Systematic genetic analysis 7. Synthetic genetic array (SGA)......ces cerevisiae and Schizosaccharo....... Quantitative genetic interaction......, Assen Roguev and Nevan J. Kroga...... 10. Exploring gene function and drug action us......al.] 11. Yeast expression proteomics by ......esper V. Olsen and Matthias Mann ......ve analysis of protein phosphorylatio......nd Bodenmiller and Ruedi Aebersold 14. A toolkit...
- 件名Yeast fungi -- Genetics. Genetic Techniques. Molecular Biology. Yeasts -- genetics.
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- 内容細目Drosophila melanogaster and the developmen......iew on raising and handling Drosophila....../ Hugo Stocker and Peter Gallant ......achel Drysdale and the FlyBase Co......transposons to generate transgenic flies / André Bachmann and Elisabeth Knus......system for the expression of genes / David A. Elliott and Andrea H. Brand P-element mutagenesis / Thomas Hummel and Christian Kläm......ens : from mutagenesis to screening and mapping / Chri......phila cells / Sandra Steinbrink and Michael Boutro......, Wei J. Gong, and Kent G. Golic Recombinases and their use in gene activation, gene inactivation, and transgenesis / Johannes Bisch...
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- 内容細目...t / Barry Dion and Grant W. Brown......pic activation and microarray expression profiling in y......igh-resolution genetic mapping in ......chael Costanzo and Charles Boone ......ased synthetic genetic array analy...... Klaus Natter, and Sepp D. Kohlwe......r J. Murakami, and Matt Kaeberlei......elucidation of gene function and genetic pathways / ......njamin Newcomb and Antonio Bedalo......ploiting yeast genetics to inform ......viano Giorgini and Paul J. Muchow......essica Jackson and Ali Shilatifar......ization of the protein interaction network and protein complexes in S......evisiae using tandem affinity purification and m...
- 件名Saccharomyces cerevisiae -- Genetics. Molecular genetics -- Techniq...... cerevisiae -- genetics -- Laboratory Manuals. Genetic Techniques ......ory Techniques and Procedures -- Laboratory Manu...
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- 内容細目...ichia pastoris and other expression systems / Jame...... Cregg Vectors and strains for expression / Joan Lin-Cereghino and Geoff P. Lin-C......ational design and optimization of fed-batch and continuous fer......, Mehmet Inan, and Michael M. Mea......pathway by overexpression of a hookworm ......or americanus) protein (Na-ASP1) / Me......n ... [et al.] Purification of the N- and C-terminal sub......resins for the purification of proteins / Sandra E. Ríos, Erin M. Giaccone, and Tillman U. Ger......arides on glycoproteins / Roger K. Br......to produce homogeneous human-like ......f heterologous proteins / Huijan Li ... [et al.] Hea...
- 件名...Biotechnology. Genetic vectors. Pichia -- genetics -- Laborat......s. Recombinant Proteins -- Laboratory Manuals.
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- 内容細目Retroviral delivery of ECM genes / Vitali alexeev and Olga Igoucheva......ific KO of ECM proteins / Mara Branca......, Emila Turco, and Emilio Hirsch ......m insect cells and yeast / Johann......rju Eukaryotic expression and purification of recombinant......ellular matrix proteins carrying the ......for functional and structural stu......revor J. Sims, and Allen J. Baile......height-mapping and nano-mechanics......Cay M. Kielty, and Michael J. She......nis Karamanos, and Nikola J. Mise......tary shadowing and transmission e......to pattern ECM proteins and cells on subst......r studying ECM protein-protein interactions / A. Pau...
- 件名...ellular Matrix Proteins -- Laboratory......ellular matrix proteins -- Laboratory manuals.
- 一般注記Previous ed.: edited by Charles H. Streuli and Michael E. Grant. Totowa, N.J...
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- 内容細目Machine generated contents ...... DNA Structure and Gene Expression 1.2. Molecular...... Regulation of Gene Expression 2.4.1. Transcr....... Single Cells and their Complexi......from Evolution and from Developme......t ch. 4 Understanding Cellular Di......tion to Cancerogenesis: Towards Ta......6.2. Detection and Malignant Orig......Cells 6.4. Oncogene Dependence and Tumor Suppress......issue Sampling and Preparation) 7......ombined Genome and Transcriptome ......Analysis ch. 9 Gene Analysis of Si......e Cells / Bertrand Lambolez 9.1. ......rrelating mRNA Expression and Functional Pro......9.4. Molecular and Functional Phenotyping of Neu...